Isolation and characterization of cytochrome b-562 from cytochrome b-c1 comlex of the photosynthetic bacterium Rhodopseudomonas sphaeroides R-26

Koh Iba, Ken ichiro Takamiya, Hiroyuki Arata

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Abstract

A b-type cytochrome (cytochrome b-562) was highly purified from the isolated cytochrome b-c1 complex of Rhodopseudomonas sphaeroides R-26 by treatment with sodium N-lauroyl sarcosinate (sarkosyi) and sodium thiocyanate, followed by gel filtration. The purified cytochrome b-562 was electrophoretically homogeneous, and its minimal Mr was estimated to be 16000 by SDS-polyacrylamide gel electrophoresis. It showed absorption maxima at 278, 415 and 534 nm in the oxidized form, and 428, 530 and 562 nm in the reduced form. Redox titration of this cytochrome was biphasic, giving two midpoint potentials, 55 and -100 mV, at pH 7.0. This indicates that the cytochrome is intact. Cytochrome b-562 Cytochrome b-C1 complex Rhodopseudomonas sphaeroides.

Original languageEnglish
Pages (from-to)151-154
Number of pages4
JournalFEBS Letters
Volume183
Issue number1
DOIs
Publication statusPublished - Apr 8 1985

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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