TY - JOUR
T1 - Isolation and characterization of cytochrome b-562 from cytochrome b-c1 comlex of the photosynthetic bacterium Rhodopseudomonas sphaeroides R-26
AU - Iba, Koh
AU - Takamiya, Ken ichiro
AU - Arata, Hiroyuki
N1 - Funding Information:
We are gratefult o Professor M. Nishimura for his valuabled iscussionsa nd suggestionsa, nd en-couragemendt uringt his study.W e alsot hankPro-fessorS . Iwanagaa nd Dr H. Kato for their help in the amino acid analysis. Part of this study was financially supportedb y a Grant-in-Aid for Scientific Research( no.59480010f)r om the Japanese Ministry of Education, Sciencea nd Culture.
PY - 1985/4/8
Y1 - 1985/4/8
N2 - A b-type cytochrome (cytochrome b-562) was highly purified from the isolated cytochrome b-c1 complex of Rhodopseudomonas sphaeroides R-26 by treatment with sodium N-lauroyl sarcosinate (sarkosyi) and sodium thiocyanate, followed by gel filtration. The purified cytochrome b-562 was electrophoretically homogeneous, and its minimal Mr was estimated to be 16000 by SDS-polyacrylamide gel electrophoresis. It showed absorption maxima at 278, 415 and 534 nm in the oxidized form, and 428, 530 and 562 nm in the reduced form. Redox titration of this cytochrome was biphasic, giving two midpoint potentials, 55 and -100 mV, at pH 7.0. This indicates that the cytochrome is intact. Cytochrome b-562 Cytochrome b-C1 complex Rhodopseudomonas sphaeroides.
AB - A b-type cytochrome (cytochrome b-562) was highly purified from the isolated cytochrome b-c1 complex of Rhodopseudomonas sphaeroides R-26 by treatment with sodium N-lauroyl sarcosinate (sarkosyi) and sodium thiocyanate, followed by gel filtration. The purified cytochrome b-562 was electrophoretically homogeneous, and its minimal Mr was estimated to be 16000 by SDS-polyacrylamide gel electrophoresis. It showed absorption maxima at 278, 415 and 534 nm in the oxidized form, and 428, 530 and 562 nm in the reduced form. Redox titration of this cytochrome was biphasic, giving two midpoint potentials, 55 and -100 mV, at pH 7.0. This indicates that the cytochrome is intact. Cytochrome b-562 Cytochrome b-C1 complex Rhodopseudomonas sphaeroides.
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U2 - 10.1016/0014-5793(85)80974-1
DO - 10.1016/0014-5793(85)80974-1
M3 - Article
AN - SCOPUS:0021952261
SN - 0014-5793
VL - 183
SP - 151
EP - 154
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -