Isolation and characterization of dibenzofuran-degrading bacteria

Kazumasa Fukuda, Sumio Nagata, Hatsumi Taniguchi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Two bacterial strains capable of utilizing dibenzofuran (DF) as a sole carbon source were isolated from soil samples of reclaimed land. The strains designated HL1 and HL7 were identified as Klebsiella sp. and Sphingomonas sp., respectively, on the basis of biochemical characteristics and the sequences of the 16S ribosomal DNA. Sphingomonas sp. strain HL7 degraded non-, mono- and also dichlorinated DF and dibenzo-p-dioxin (DD). Klebsiella sp. strain HL1 was able to degrade non- and monochlorinated DFs and DDs, but not dichlorinated ones. The metabolites formed from DF by strains HL1 and HL7 were similar to those by dioxin-degrading bacteria Sphingomonas sp. strain RW1 except for salicylic acid and catechol. Strain HL7 had a gene homologous to that encoding the dioxin dioxygenase alpha-subunit (dxnA1) gene of Sphingomonas sp. strain RW1. However, Southern hybridization analysis showed that the size of an EcoRV-digested genomic fragment involving the dioxin dioxygenase gene of strain HL7 was smaller than that of strain RW1, and that strain HL1 did not have the homologous gene. Strains HL1 and HL7 provided useful information regarding the dioxygenase genes.

Original languageEnglish
Pages (from-to)179-185
Number of pages7
JournalFEMS microbiology letters
Volume208
Issue number2
DOIs
Publication statusPublished - Mar 5 2002
Externally publishedYes

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Sphingomonas
Bacteria
Klebsiella
Genes
Dioxygenases
Dioxins
Salicylic Acid
Ribosomal DNA
Soil
Carbon
dibenzofuran
dioxin dioxygenase

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Isolation and characterization of dibenzofuran-degrading bacteria. / Fukuda, Kazumasa; Nagata, Sumio; Taniguchi, Hatsumi.

In: FEMS microbiology letters, Vol. 208, No. 2, 05.03.2002, p. 179-185.

Research output: Contribution to journalArticle

Fukuda, Kazumasa ; Nagata, Sumio ; Taniguchi, Hatsumi. / Isolation and characterization of dibenzofuran-degrading bacteria. In: FEMS microbiology letters. 2002 ; Vol. 208, No. 2. pp. 179-185.
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