Isolation and characterization of differently expressed cDNAs in a meiotic recombination strain of Bombyx mori

Yoshitaka Miyagawa, Takahiro Kusakabe, Jae Man Lee, Takuji Maeda, Yutaka Kawaguchi, Katsumi Koga

Research output: Contribution to journalArticle

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Abstract

The male gametogenesis is a complex process that is influenced by various components. In some Bombyx mori strains, the remarkabley reduced meiotic recombination rates had been observed when they were calculated based on the frequency of crossing over between two marker genes, Striped (ps) and Yellow blood (Y). We have employed a differential display analysis of testis transcripts and isolated two clones that were down regulated in the r20 strain, wherein low recombination is maintained. The RT-PCR analysis revealed that the expression of relevant genes was not restricted in the testis. Also the developmental profiles of the expression of these genes in the testis were examined; one of the transcripts, termed BmPI-T1, was expressed during the larval and pupal stage, but disappeared at the adult stage. The other transcript, termed BmLSP-T, was strongly expressed on day 3 to 5 of the fifth instar. The BmPI-T1 and BmLSP-T mRNAs contained open reading frames of 1086 and 801 nucleotides in length, encoding 362 and 267 amino acid residues, respectively. The former resembles the membrane transport facilitator superfamily and the latter is akin to the 30 kDa proteins. The current proteins are novel candidates that may be involved in metabolic events during the male gametegenesis.

Original languageEnglish
Pages (from-to)117-127
Number of pages11
JournalJournal of Insect Biotechnology and Sericology
Volume73
Issue number3
Publication statusPublished - Oct 1 2004

Fingerprint

Bombyx
Bombyx mori
Genetic Recombination
Testis
testes
Complementary DNA
Genes
Gametogenesis
Proteins
gene expression
crossing over
gametogenesis
Nucleotides
Transcriptome
Open Reading Frames
open reading frames
Amino acids
Blood
instars
proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

Cite this

Isolation and characterization of differently expressed cDNAs in a meiotic recombination strain of Bombyx mori. / Miyagawa, Yoshitaka; Kusakabe, Takahiro; Lee, Jae Man; Maeda, Takuji; Kawaguchi, Yutaka; Koga, Katsumi.

In: Journal of Insect Biotechnology and Sericology, Vol. 73, No. 3, 01.10.2004, p. 117-127.

Research output: Contribution to journalArticle

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