Isolation and characterization of enterocin SE-K4 produced by thermophilic enterococci, Enterococcus faecalis K-4

Tomoko Eguchi, Kazuyo Kaminaka, Jun Shima, Shinichi Kawamoto, Katsumi Mori, Seong Hyun Choi, Katsumi Doi, Sadahiro Ohmomo, Seiya Ogata

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Enterococcus sp. K-4, with a bacteriocin-like activity against E. faecium, was isolated from grass silage in Thailand. Morphological, physiological, and phylogenetic studies clearly identified strain K-4 as a strain of E. faecalis. Strain K-4 produced a maximal amount of bacteriocin at 43-45°C. We purified, for the first time, the bacteriocin produced at high temperature by E. faecalis to homogeneity, using adsorption on cells of the producer strain and reversed-phase liquid chromatography. The bacteriocin, designated enterocin SE-K4, is a peptide of about 5 kDa as measured by SDS-PAGE, and Mass spectrometry analysis found the molecular mass of 5356.2, which is in good agreement. The amino acid sequencing of the N-terminal end of enterocin SE-K4 showed apparent sequence similarity to class IIa bacteriocins. Enterocin SE-K4 was active against E. faecium, E. faecalis, Bacillus subtilis, Clostridium beijerinckii, and Listeria monocytogenes. Enterocin SE-K4 is very heat stable.

Original languageEnglish
Pages (from-to)247-253
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume65
Issue number2
DOIs
Publication statusPublished - Feb 1 2001

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Bacteriocins
Enterococcus faecalis
Enterococcus
Clostridium beijerinckii
Listeria
Clostridium
Silage
Liquid chromatography
Protein Sequence Analysis
Listeria monocytogenes
Molecular mass
Bacilli
Thailand
Reverse-Phase Chromatography
Poaceae
Bacillus subtilis
Adsorption
Mass spectrometry
Polyacrylamide Gel Electrophoresis
Mass Spectrometry

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Isolation and characterization of enterocin SE-K4 produced by thermophilic enterococci, Enterococcus faecalis K-4. / Eguchi, Tomoko; Kaminaka, Kazuyo; Shima, Jun; Kawamoto, Shinichi; Mori, Katsumi; Choi, Seong Hyun; Doi, Katsumi; Ohmomo, Sadahiro; Ogata, Seiya.

In: Bioscience, Biotechnology and Biochemistry, Vol. 65, No. 2, 01.02.2001, p. 247-253.

Research output: Contribution to journalArticle

Eguchi, Tomoko ; Kaminaka, Kazuyo ; Shima, Jun ; Kawamoto, Shinichi ; Mori, Katsumi ; Choi, Seong Hyun ; Doi, Katsumi ; Ohmomo, Sadahiro ; Ogata, Seiya. / Isolation and characterization of enterocin SE-K4 produced by thermophilic enterococci, Enterococcus faecalis K-4. In: Bioscience, Biotechnology and Biochemistry. 2001 ; Vol. 65, No. 2. pp. 247-253.
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