Isolation and characterization of Fetal Leydig progenitor cells of male mice

Miki Inoue, Yuichi Shima, Kanako Miyabayashi, Kaori Tokunaga, Tetsuya Sato, Takashi Baba, Yasuyuki Ohkawa, Haruhiko Akiyama, Mikita Suyama, Ken Ichirou Morohashi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Fetal and adult Leydig cells develop in mammalian prenatal and postnatal testes, respectively. In mice, fetal Leydig cells (FLCs) emergeintheinterstitial spaceofthe testisatembryonic day 12.5 and thereafter increase in number, possibly through differentiation from progenitor cells. However, the progenitor cells have not yet been identified. Previously, we established transgenic mice in which FLCs are labeled strongly with enhanced green fluorescent protein (EGFP). Interestingly, fluorescence-activated cell sorting provided us with weakly EGFP-labeled cells as well as strongly EGFP-labeled FLCs. In vitro reconstruction of fetal testes demonstrated that weakly EGFP-labeled cells contain FLC progenitors. Transcriptome from the 2 cell populations revealed, as expected, marked differences in the expression of genes required for growth factor/receptor signaling and steroidogenesis. In addition, genes for energy metabolisms such as glycolytic pathways and the citrate cycle were activated in strongly EGFP-labeled cells, suggesting that metabolism is activated during FLC differentiation.

Original languageEnglish
Pages (from-to)1222-1233
Number of pages12
JournalEndocrinology
Volume157
Issue number3
DOIs
Publication statusPublished - Mar 2016

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Leydig Cells
Stem Cells
Testis
Growth Factor Receptors
Transcriptome
Citric Acid
Energy Metabolism
Transgenic Mice
Cell Differentiation
Flow Cytometry
enhanced green fluorescent protein
Gene Expression
Population
Genes

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Isolation and characterization of Fetal Leydig progenitor cells of male mice. / Inoue, Miki; Shima, Yuichi; Miyabayashi, Kanako; Tokunaga, Kaori; Sato, Tetsuya; Baba, Takashi; Ohkawa, Yasuyuki; Akiyama, Haruhiko; Suyama, Mikita; Morohashi, Ken Ichirou.

In: Endocrinology, Vol. 157, No. 3, 03.2016, p. 1222-1233.

Research output: Contribution to journalArticle

Inoue M, Shima Y, Miyabayashi K, Tokunaga K, Sato T, Baba T et al. Isolation and characterization of Fetal Leydig progenitor cells of male mice. Endocrinology. 2016 Mar;157(3):1222-1233. https://doi.org/10.1210/en.2015-1773
Inoue, Miki ; Shima, Yuichi ; Miyabayashi, Kanako ; Tokunaga, Kaori ; Sato, Tetsuya ; Baba, Takashi ; Ohkawa, Yasuyuki ; Akiyama, Haruhiko ; Suyama, Mikita ; Morohashi, Ken Ichirou. / Isolation and characterization of Fetal Leydig progenitor cells of male mice. In: Endocrinology. 2016 ; Vol. 157, No. 3. pp. 1222-1233.
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