Isolation and characterization of hardening-induced proteins in Chlorella vulgaris c-27: Identification of late embryogenesis abundant proteins

Ken-ichi Honjoh, Makoto Yoshimoto, Toshio Joh, Taishin Kajiwara, Takahisa Miyamoto, Shoji Hatano

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Hardening-induced soluble proteins of Chlorella vulgaris Beijerink IAM C-27 (formerly Chlorella ellipsoidea Gerneck IAM C-27) were isolated and purified by two-dimensional high-performance liquid chromatography (2D-HPLC) on an anion-exchange column, with subsequent reversed-phase chromatography. Some of the proteins were resolved by SDS-PAGE, characterized by amino-terminal sequencing and identified by searching for homologies in databases. Separation of the soluble proteins during the hardening of Chlorella by a combination of 2D-HPLC and SDS-PAGE revealed that at least 31 proteins were induced or increased in abundance. Of particular interest was the induction after 12 h of a 10-kDa protein with the amino-terminal amino acid sequence AGNKPITEQISDAVGAAGQKVG and the induction after 6 h of a 14-kDa protein with the amino-terminal sequence ALGEESLGDKAKNAFEDAKDAVKDAAGNVKEAV. The amino-terminal sequences of these proteins indicated that they were homologous to late embryogenesis abundant (LEA) proteins. Furthermore, the level of a 22-kDa protein also increased after 12 h. The amino-terminal sequence of this protein, AAPLVGGPAPDFTAAAVFD, indicated that it was homologous to thioredoxin peroxidase.

Original languageEnglish
Pages (from-to)1421-1430
Number of pages10
JournalPlant and Cell Physiology
Volume36
Issue number8
Publication statusPublished - Dec 1 1995

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Chlorella vulgaris
Embryonic Development
embryogenesis
Proteins
proteins
amino acid sequences
high performance liquid chromatography
Chlorella
High Pressure Liquid Chromatography
polyacrylamide gel electrophoresis
Chlorella ellipsoidea
Polyacrylamide Gel Electrophoresis
peroxiredoxin
Peroxiredoxins
anion exchange
Reverse-Phase Chromatography
chromatography
Anions
Amino Acid Sequence

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Isolation and characterization of hardening-induced proteins in Chlorella vulgaris c-27 : Identification of late embryogenesis abundant proteins. / Honjoh, Ken-ichi; Yoshimoto, Makoto; Joh, Toshio; Kajiwara, Taishin; Miyamoto, Takahisa; Hatano, Shoji.

In: Plant and Cell Physiology, Vol. 36, No. 8, 01.12.1995, p. 1421-1430.

Research output: Contribution to journalArticle

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