Abstract
In Japan, indigenous tick-borne phleboviruses (TBPVs) and their associated diseases first became evident in 2013 by reported human cases of severe fever with thrombocytopenia syndrome (SFTS). In this study, we report a novel member of the genus Phlebovirus designated as Kabuto Mountain virus (KAMV), which was isolated from the ixodid tick Haemaphysalis flava in Hyogo, Japan. A complete viral genome sequencing and phylogenetic analyses showed that KAMV is a novel member of TBPVs, which is closely related to the Uukuniemi and Kaisodi group viruses. However, unlike the Uukuniemi group viruses, the 165-nt intergenic region (IGR) in the KAMV S segment was highly C-rich in the genomic sense and not predicted to form a secondary structure, which are rather similar to those of the Kaisodi group viruses and most mosquito/sandfly-borne phleboviruses. Furthermore, the NSs protein of KAMV was highly divergent from those of other TBPVs. These results provided further insights into the genetic diversity and evolutionary relationships of TBPVs. KAMV could infect and replicate in some rodent and primate cell lines. We evaluated the infectivity and pathogenicity of KAMV in suckling mice, where we obtained a virulent strain after two passages via intracerebral inoculation. This is the first report showing the existence of a previously unrecognized TBPV in Japan, other than the SFTS virus.
| Original language | English |
|---|---|
| Pages (from-to) | 252-261 |
| Number of pages | 10 |
| Journal | Virus Research |
| Volume | 244 |
| DOIs | |
| Publication status | Published - Jan 15 2018 |
| Externally published | Yes |
Fingerprint
All Science Journal Classification (ASJC) codes
- Virology
- Infectious Diseases
- Cancer Research
Cite this
Isolation and characterization of Kabuto Mountain virus, a new tick-borne phlebovirus from Haemaphysalis flava ticks in Japan. / Ejiri, Hiroko; Lim, Chang Kweng; Isawa, Haruhiko; Yamaguchi, Yukie; Fujita, Ryosuke; Takayama-Ito, Mutsuyo; Kuwata, Ryusei; Kobayashi, Daisuke; Horiya, Madoka; Posadas-Herrera, Guillermo; Iizuka-Shiota, Itoe; Kakiuchi, Satsuki; Katayama, Yukie; Hayashi, Toshihiko; Sasaki, Toshinori; Kobayashi, Mutsuo; Morikawa, Shigeru; Maeda, Ken; Mizutani, Tetsuya; Kaku, Koki; Saijo, Masayuki; Sawabe, Kyoko.
In: Virus Research, Vol. 244, 15.01.2018, p. 252-261.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and characterization of Kabuto Mountain virus, a new tick-borne phlebovirus from Haemaphysalis flava ticks in Japan
AU - Ejiri, Hiroko
AU - Lim, Chang Kweng
AU - Isawa, Haruhiko
AU - Yamaguchi, Yukie
AU - Fujita, Ryosuke
AU - Takayama-Ito, Mutsuyo
AU - Kuwata, Ryusei
AU - Kobayashi, Daisuke
AU - Horiya, Madoka
AU - Posadas-Herrera, Guillermo
AU - Iizuka-Shiota, Itoe
AU - Kakiuchi, Satsuki
AU - Katayama, Yukie
AU - Hayashi, Toshihiko
AU - Sasaki, Toshinori
AU - Kobayashi, Mutsuo
AU - Morikawa, Shigeru
AU - Maeda, Ken
AU - Mizutani, Tetsuya
AU - Kaku, Koki
AU - Saijo, Masayuki
AU - Sawabe, Kyoko
PY - 2018/1/15
Y1 - 2018/1/15
N2 - In Japan, indigenous tick-borne phleboviruses (TBPVs) and their associated diseases first became evident in 2013 by reported human cases of severe fever with thrombocytopenia syndrome (SFTS). In this study, we report a novel member of the genus Phlebovirus designated as Kabuto Mountain virus (KAMV), which was isolated from the ixodid tick Haemaphysalis flava in Hyogo, Japan. A complete viral genome sequencing and phylogenetic analyses showed that KAMV is a novel member of TBPVs, which is closely related to the Uukuniemi and Kaisodi group viruses. However, unlike the Uukuniemi group viruses, the 165-nt intergenic region (IGR) in the KAMV S segment was highly C-rich in the genomic sense and not predicted to form a secondary structure, which are rather similar to those of the Kaisodi group viruses and most mosquito/sandfly-borne phleboviruses. Furthermore, the NSs protein of KAMV was highly divergent from those of other TBPVs. These results provided further insights into the genetic diversity and evolutionary relationships of TBPVs. KAMV could infect and replicate in some rodent and primate cell lines. We evaluated the infectivity and pathogenicity of KAMV in suckling mice, where we obtained a virulent strain after two passages via intracerebral inoculation. This is the first report showing the existence of a previously unrecognized TBPV in Japan, other than the SFTS virus.
AB - In Japan, indigenous tick-borne phleboviruses (TBPVs) and their associated diseases first became evident in 2013 by reported human cases of severe fever with thrombocytopenia syndrome (SFTS). In this study, we report a novel member of the genus Phlebovirus designated as Kabuto Mountain virus (KAMV), which was isolated from the ixodid tick Haemaphysalis flava in Hyogo, Japan. A complete viral genome sequencing and phylogenetic analyses showed that KAMV is a novel member of TBPVs, which is closely related to the Uukuniemi and Kaisodi group viruses. However, unlike the Uukuniemi group viruses, the 165-nt intergenic region (IGR) in the KAMV S segment was highly C-rich in the genomic sense and not predicted to form a secondary structure, which are rather similar to those of the Kaisodi group viruses and most mosquito/sandfly-borne phleboviruses. Furthermore, the NSs protein of KAMV was highly divergent from those of other TBPVs. These results provided further insights into the genetic diversity and evolutionary relationships of TBPVs. KAMV could infect and replicate in some rodent and primate cell lines. We evaluated the infectivity and pathogenicity of KAMV in suckling mice, where we obtained a virulent strain after two passages via intracerebral inoculation. This is the first report showing the existence of a previously unrecognized TBPV in Japan, other than the SFTS virus.
UR - http://www.scopus.com/inward/record.url?scp=85036620479&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85036620479&partnerID=8YFLogxK
U2 - 10.1016/j.virusres.2017.11.030
DO - 10.1016/j.virusres.2017.11.030
M3 - Article
C2 - 29197549
AN - SCOPUS:85036620479
VL - 244
SP - 252
EP - 261
JO - Virus Research
JF - Virus Research
SN - 0168-1702
ER -