Abstract
Microsatellites were isolated and characterized for Japanese larch, Larix kaempferi, a conifer species distributed in Japan. A larch genomic DNA library enriched for (AG)n repeats was screened using the colony polymerase chain reaction method and 145 unique microsatellite containing sequences were obtained. Seventy-two primer pairs were designed and 30 produced single-locus products, and 19 of them were polymorphic. The expected heterozygosity ranged from 0.566 to 0.951. These 19 polymorphic microsatellite loci should be valuable markers for genetic studies on Japanese larch.
| Original language | English |
|---|---|
| Pages (from-to) | 664-666 |
| Number of pages | 3 |
| Journal | Molecular Ecology Notes |
| Volume | 6 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sep 2006 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Ecology
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
Cite this
Isolation and characterization of microsatellite loci from Larix kaempferi. / Isoda, Keiya; Watanabe, Atsushi.
In: Molecular Ecology Notes, Vol. 6, No. 3, 09.2006, p. 664-666.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and characterization of microsatellite loci from Larix kaempferi
AU - Isoda, Keiya
AU - Watanabe, Atsushi
PY - 2006/9
Y1 - 2006/9
N2 - Microsatellites were isolated and characterized for Japanese larch, Larix kaempferi, a conifer species distributed in Japan. A larch genomic DNA library enriched for (AG)n repeats was screened using the colony polymerase chain reaction method and 145 unique microsatellite containing sequences were obtained. Seventy-two primer pairs were designed and 30 produced single-locus products, and 19 of them were polymorphic. The expected heterozygosity ranged from 0.566 to 0.951. These 19 polymorphic microsatellite loci should be valuable markers for genetic studies on Japanese larch.
AB - Microsatellites were isolated and characterized for Japanese larch, Larix kaempferi, a conifer species distributed in Japan. A larch genomic DNA library enriched for (AG)n repeats was screened using the colony polymerase chain reaction method and 145 unique microsatellite containing sequences were obtained. Seventy-two primer pairs were designed and 30 produced single-locus products, and 19 of them were polymorphic. The expected heterozygosity ranged from 0.566 to 0.951. These 19 polymorphic microsatellite loci should be valuable markers for genetic studies on Japanese larch.
UR - http://www.scopus.com/inward/record.url?scp=33747383860&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33747383860&partnerID=8YFLogxK
U2 - 10.1111/j.1471-8286.2006.01291.x
DO - 10.1111/j.1471-8286.2006.01291.x
M3 - Article
VL - 6
SP - 664
EP - 666
JO - Molecular Ecology Resources
JF - Molecular Ecology Resources
SN - 1755-098X
IS - 3
ER -