Abstract
Microsatellites were isolated and characterized for Japanese larch, Larix kaempferi, a conifer species distributed in Japan. A larch genomic DNA library enriched for (AG)n repeats was screened using the colony polymerase chain reaction method and 145 unique microsatellite containing sequences were obtained. Seventy-two primer pairs were designed and 30 produced single-locus products, and 19 of them were polymorphic. The expected heterozygosity ranged from 0.566 to 0.951. These 19 polymorphic microsatellite loci should be valuable markers for genetic studies on Japanese larch.
Original language | English |
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Pages (from-to) | 664-666 |
Number of pages | 3 |
Journal | Molecular Ecology Notes |
Volume | 6 |
Issue number | 3 |
DOIs | |
Publication status | Published - Sep 2006 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Ecology
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry