Isolation and characterization of microsatellite loci from Larix kaempferi

Keiya Isoda, Atsushi Watanabe

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Microsatellites were isolated and characterized for Japanese larch, Larix kaempferi, a conifer species distributed in Japan. A larch genomic DNA library enriched for (AG)n repeats was screened using the colony polymerase chain reaction method and 145 unique microsatellite containing sequences were obtained. Seventy-two primer pairs were designed and 30 produced single-locus products, and 19 of them were polymorphic. The expected heterozygosity ranged from 0.566 to 0.951. These 19 polymorphic microsatellite loci should be valuable markers for genetic studies on Japanese larch.

Original languageEnglish
Pages (from-to)664-666
Number of pages3
JournalMolecular Ecology Notes
Volume6
Issue number3
DOIs
Publication statusPublished - Sep 2006
Externally publishedYes

Fingerprint

Larix
Larix kaempferi
heterozygosity
Microsatellite Repeats
polymerase chain reaction
coniferous tree
genomics
microsatellite repeats
DNA
loci
DNA libraries
Polymerase chain reaction
Coniferophyta
Gene Library
conifers
Genomic Library
Genetic Markers
Japan
genetic markers
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Isolation and characterization of microsatellite loci from Larix kaempferi. / Isoda, Keiya; Watanabe, Atsushi.

In: Molecular Ecology Notes, Vol. 6, No. 3, 09.2006, p. 664-666.

Research output: Contribution to journalArticle

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