Isolation and characterization of microsatellite loci from Larix kaempferi

Keiya Isoda; Atsushi Watanabe

doi:10.1111/j.1471-8286.2006.01291.x

Isolation and characterization of microsatellite loci from Larix kaempferi

Keiya Isoda, Atsushi Watanabe

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Microsatellites were isolated and characterized for Japanese larch, Larix kaempferi, a conifer species distributed in Japan. A larch genomic DNA library enriched for (AG)_n repeats was screened using the colony polymerase chain reaction method and 145 unique microsatellite containing sequences were obtained. Seventy-two primer pairs were designed and 30 produced single-locus products, and 19 of them were polymorphic. The expected heterozygosity ranged from 0.566 to 0.951. These 19 polymorphic microsatellite loci should be valuable markers for genetic studies on Japanese larch.

Original language	English
Pages (from-to)	664-666
Number of pages	3
Journal	Molecular Ecology Notes
Volume	6
Issue number	3
DOIs	https://doi.org/10.1111/j.1471-8286.2006.01291.x
Publication status	Published - Sep 2006
Externally published	Yes

All Science Journal Classification (ASJC) codes

Ecology
Biochemistry, Genetics and Molecular Biology(all)
Biochemistry

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AB - Microsatellites were isolated and characterized for Japanese larch, Larix kaempferi, a conifer species distributed in Japan. A larch genomic DNA library enriched for (AG)n repeats was screened using the colony polymerase chain reaction method and 145 unique microsatellite containing sequences were obtained. Seventy-two primer pairs were designed and 30 produced single-locus products, and 19 of them were polymorphic. The expected heterozygosity ranged from 0.566 to 0.951. These 19 polymorphic microsatellite loci should be valuable markers for genetic studies on Japanese larch.

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