Isolation and characterization of microsatellite loci from Quercus mongolica var. crispula

Kentaro Mishima; Atsushi Watanabe; Keiya Isoda; Masatoshi Ubukata; Katsuhiko Takata

doi:10.1111/j.1471-8286.2006.01313.x

Isolation and characterization of microsatellite loci from Quercus mongolica var. crispula

Kentaro Mishima, Atsushi Watanabe, Keiya Isoda, Masatoshi Ubukata, Katsuhiko Takata

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Microsatellites were isolated and characterized for the Japanese oak species, Quercus mongolica var. crispula, distributed in temperate deciduous forests of Japan. Eleven of the 48 primer pairs designed successfully amplified unambiguous and polymorphic single loci among 67 Q. mongolica var. crispula individuals within a plot in southwestern Japan. The observed and expected heterozygosities of the 11 microsatellite markers ranged from 0.522 to 0.896 and from 0.536 to 0.882, respectively. These polymorphic microsatellite markers are useful for estimating pollen-mediated gene flow in Q. mongolica var. crispula.

Original language	English
Pages (from-to)	695-697
Number of pages	3
Journal	Molecular Ecology Notes
Volume	6
Issue number	3
DOIs	https://doi.org/10.1111/j.1471-8286.2006.01313.x
Publication status	Published - Sept 1 2006
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Ecology
Biochemistry, Genetics and Molecular Biology(all)

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10.1111/j.1471-8286.2006.01313.x

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abstract = "Microsatellites were isolated and characterized for the Japanese oak species, Quercus mongolica var. crispula, distributed in temperate deciduous forests of Japan. Eleven of the 48 primer pairs designed successfully amplified unambiguous and polymorphic single loci among 67 Q. mongolica var. crispula individuals within a plot in southwestern Japan. The observed and expected heterozygosities of the 11 microsatellite markers ranged from 0.522 to 0.896 and from 0.536 to 0.882, respectively. These polymorphic microsatellite markers are useful for estimating pollen-mediated gene flow in Q. mongolica var. crispula.",

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AU - Mishima, Kentaro

AU - Watanabe, Atsushi

AU - Isoda, Keiya

AU - Ubukata, Masatoshi

AU - Takata, Katsuhiko

PY - 2006/9/1

Y1 - 2006/9/1

N2 - Microsatellites were isolated and characterized for the Japanese oak species, Quercus mongolica var. crispula, distributed in temperate deciduous forests of Japan. Eleven of the 48 primer pairs designed successfully amplified unambiguous and polymorphic single loci among 67 Q. mongolica var. crispula individuals within a plot in southwestern Japan. The observed and expected heterozygosities of the 11 microsatellite markers ranged from 0.522 to 0.896 and from 0.536 to 0.882, respectively. These polymorphic microsatellite markers are useful for estimating pollen-mediated gene flow in Q. mongolica var. crispula.

AB - Microsatellites were isolated and characterized for the Japanese oak species, Quercus mongolica var. crispula, distributed in temperate deciduous forests of Japan. Eleven of the 48 primer pairs designed successfully amplified unambiguous and polymorphic single loci among 67 Q. mongolica var. crispula individuals within a plot in southwestern Japan. The observed and expected heterozygosities of the 11 microsatellite markers ranged from 0.522 to 0.896 and from 0.536 to 0.882, respectively. These polymorphic microsatellite markers are useful for estimating pollen-mediated gene flow in Q. mongolica var. crispula.

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JO - Molecular Ecology Notes

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Isolation and characterization of microsatellite loci from Quercus mongolica var. crispula

Abstract

All Science Journal Classification (ASJC) codes

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