Isolation and characterization of microsatellite markers for Thujopsis dolabrata var. hondai (Cupressaceae)

Kentaro Mishima, Tomonori Hirao, Atsushi Watanabe, Katsuhiko Takata

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Premise of the study: Microsatellite markers were developed for the endemic Japanese species Thujopsis dolabrata var. hondai for studies on forest ecology and tree breeding. Methods and Results: We characterized 10 dinucleotide microsatellite loci by screening primers developed using a simple sequence repeat-enriched library. The number of alleles per locus ranged from eight to 44 with an average of 20.3. The observed and expected heterozygosities ranged from 0.326 to 0.854 and from 0.670 to 0.976, respectively. Conclusions: The development of these markers can be used to assess useful genetic information for ecological studies and tree breeding in T. dolabrata var. hondai.

Original languageEnglish
JournalAmerican Journal of Botany
Volume99
Issue number8
DOIs
Publication statusPublished - Aug 1 2012
Externally publishedYes

Fingerprint

Thujopsis dolabrata
Cupressaceae
Microsatellite Repeats
tree breeding
breeding
microsatellite repeats
Breeding
heterozygosity
forest ecology
allele
loci
Ecology
forest trees
Libraries
Alleles
screening
alleles
marker
methodology

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Plant Science

Cite this

Isolation and characterization of microsatellite markers for Thujopsis dolabrata var. hondai (Cupressaceae). / Mishima, Kentaro; Hirao, Tomonori; Watanabe, Atsushi; Takata, Katsuhiko.

In: American Journal of Botany, Vol. 99, No. 8, 01.08.2012.

Research output: Contribution to journalArticle

@article{6f3b268fbce84bc4b304c8cc2000ee5e,
title = "Isolation and characterization of microsatellite markers for Thujopsis dolabrata var. hondai (Cupressaceae)",
abstract = "Premise of the study: Microsatellite markers were developed for the endemic Japanese species Thujopsis dolabrata var. hondai for studies on forest ecology and tree breeding. Methods and Results: We characterized 10 dinucleotide microsatellite loci by screening primers developed using a simple sequence repeat-enriched library. The number of alleles per locus ranged from eight to 44 with an average of 20.3. The observed and expected heterozygosities ranged from 0.326 to 0.854 and from 0.670 to 0.976, respectively. Conclusions: The development of these markers can be used to assess useful genetic information for ecological studies and tree breeding in T. dolabrata var. hondai.",
author = "Kentaro Mishima and Tomonori Hirao and Atsushi Watanabe and Katsuhiko Takata",
year = "2012",
month = "8",
day = "1",
doi = "10.3732/ajb.1200010",
language = "English",
volume = "99",
journal = "American Journal of Botany",
issn = "0002-9122",
publisher = "Botanical Society of America Inc.",
number = "8",

}

TY - JOUR

T1 - Isolation and characterization of microsatellite markers for Thujopsis dolabrata var. hondai (Cupressaceae)

AU - Mishima, Kentaro

AU - Hirao, Tomonori

AU - Watanabe, Atsushi

AU - Takata, Katsuhiko

PY - 2012/8/1

Y1 - 2012/8/1

N2 - Premise of the study: Microsatellite markers were developed for the endemic Japanese species Thujopsis dolabrata var. hondai for studies on forest ecology and tree breeding. Methods and Results: We characterized 10 dinucleotide microsatellite loci by screening primers developed using a simple sequence repeat-enriched library. The number of alleles per locus ranged from eight to 44 with an average of 20.3. The observed and expected heterozygosities ranged from 0.326 to 0.854 and from 0.670 to 0.976, respectively. Conclusions: The development of these markers can be used to assess useful genetic information for ecological studies and tree breeding in T. dolabrata var. hondai.

AB - Premise of the study: Microsatellite markers were developed for the endemic Japanese species Thujopsis dolabrata var. hondai for studies on forest ecology and tree breeding. Methods and Results: We characterized 10 dinucleotide microsatellite loci by screening primers developed using a simple sequence repeat-enriched library. The number of alleles per locus ranged from eight to 44 with an average of 20.3. The observed and expected heterozygosities ranged from 0.326 to 0.854 and from 0.670 to 0.976, respectively. Conclusions: The development of these markers can be used to assess useful genetic information for ecological studies and tree breeding in T. dolabrata var. hondai.

UR - http://www.scopus.com/inward/record.url?scp=84865021205&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865021205&partnerID=8YFLogxK

U2 - 10.3732/ajb.1200010

DO - 10.3732/ajb.1200010

M3 - Article

VL - 99

JO - American Journal of Botany

JF - American Journal of Botany

SN - 0002-9122

IS - 8

ER -