Isolation and characterization of microsatellite markers from Robinia pseudoacacia L.

Kentaro Mishima, Tomonori Hirao, Shougo Urano, Atsushi Watanabe, Katsuhiko Takata

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Microsatellite markers were isolated from Robinia pseudoacacia L. using an enrichment method. Eleven of the 23 primer pairs designed successfully amplified unambiguous and polymorphic single loci among 39 individual R. pseudoacacia L. from northeastern Japan. The observed and expected heterozygosities of the 11 microsatellite markers ranged from 0.333 to 0.821 and from 0.489 to 0.867, respectively. The polymorphisms observed at the 11 microsatellite loci are useful genetic data for forest ecological studies involving R. pseudoacacia L.

Original languageEnglish
Pages (from-to)850-852
Number of pages3
JournalMolecular Ecology Resources
Volume9
Issue number3
DOIs
Publication statusPublished - May 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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