Isolation and characterization of porcine endothelin-like peptide

Osamu Nakagawa, Kazuwa Nakao, Yoshihiko Saito, Gotaro Shirakami, Michihisa Jougasaki, Masashi Mukoyama, Kiminori Hosoda, Shin ichi Suga, Yoshihiro Ogawa, Ichiro Kishimoto, Yasato Komatsu, Norio Hama, Hiroo Imura

Research output: Contribution to journalArticlepeer-review

Abstract

Two endogenous molecules possessing an endothelin-like sequence with an apparent molecular weight of 7 kDa were isolated from serum-free culture medium of porcine aortic endothelial cells, using a specific radioimmunoassay. N-terminal sequences of two molecules were identical to each other and were Ser-Leu-Lys-Asp-Leu-Phe-Pro-Ala-Lys-Ala-Ala-Asp-Arg-Arg-Asp-Arg-X-Gln-X-Ala-X-Gln-Lys-Asp, which corresponds to the sequence [94-117] of preproendothelin-1. This finding indicates that these two molecules may be closely related peptides such as the oxidized form or disulfide analogues and also suggests that the endogenous peptide possessing an endothelin-like sequence is generated by proteolytic cleavage at paired basic amino acids Arg92-Arg93Further studies on the structure and function of new endogenous peptides possessing an endothelin-like sequence are ongoing in our laboratory.

Original languageEnglish
Pages (from-to)S13-S16
JournalJournal of Cardiovascular Pharmacology
Volume17
DOIs
Publication statusPublished - 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

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