Isolation and characterization of sex chromosome rearrangements generating male muscle dystrophy and female abnormal oogenesis in the silkworm, Bombyx mori

T. Fujii, T. Yokoyama, O. Ninagi, K. Kakehashi, Y. Obara, M. Nenoi, T. Ishikawa, K. Mita, T. Shimada, H. Abe

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In deletion-mapping of W-specific RAPD (W-RAPD) markers and putative female determinant gene (Fem), we used X-ray irradiation to break the translocation-carrying W chromosome (W Ze ). We succeeded in obtaining a fragment of the W Ze chromosome designated as Ze W, having 3 of 12 W-RAPD markers (W-Bonsai, W-Yukemuri-S, W-Yukemuri-L). Inheritance of the Ze W fragment by males indicates that it does not include the Fem gene. On the basis of these results, we determined the relative positions of W-Yukemuri-S and W-Yukemuri-L, and we narrowed down the region where Fem gene is located. In addition to the Ze W fragment, the Z chromosome was also broken into a large fragment (Z1) having the + sch (1-21.5) and a small fragment (Z2) having the + od (1-49.6). Moreover, a new chromosomal fragment (Ze WZ2) was generated by a fusion event between the Ze W and the Z2 fragments. We analyzed the genetic behavior of the Z1 fragment and the Ze WZ2 fragment during male (Z/Z1 Ze WZ2) and female (Z1 Ze WZ2/W) meiosis using phenotypic markers. It was observed that the Z1 fragment and the Z or the W chromosomes separate without fail. On the other hand, non-disjunction between the Ze WZ2 fragment and the Z chromosome and also between the Ze WZ2 fragment and the W chromosome occurred. Furthermore, the females (2A: Z/Ze WZ2/W) and males (2A: Z/Z 1) resulting from non-disjunction between the Ze WZ 2 fragment and the W chromosome had phenotypic defects: namely, females exhibited abnormal oogenesis and males were flapless due to abnormal indirect flight muscle structure. These results suggest that Z2 region of the Z chromosome contains dose-sensitive gene(s), which are involved in oogenesis and indirect flight muscle development.

Original languageEnglish
Pages (from-to)267-280
Number of pages14
JournalGenetica
Volume130
Issue number3
DOIs
Publication statusPublished - Jul 1 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Genetics
  • Plant Science
  • Insect Science

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