Isolation and characterization of Tarumizu tick virus

A new coltivirus from Haemaphysalis flava ticks in Japan

Ryosuke Fujita, Hiroko Ejiri, Chang Kweng Lim, Shinichi Noda, Takeo Yamauchi, Mamoru Watanabe, Daisuke Kobayashi, Mutsuyo Takayama-Ito, Katsunori Murota, Guillermo Posadas-Herrera, Shohei Minami, Ryusei Kuwata, Yukie Yamaguchi, Madoka Horiya, Yukie Katayama, Hiroshi Shimoda, Masayuki Saijo, Ken Maeda, Tetsuya Mizutani, Haruhiko Isawa & 1 others Kyoko Sawabe

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

During the course of tick-borne virus surveillance in Japan, three independent isolates of probably the same virus were obtained from three geographically distant populations of the hard tick Haemaphysalis flava. Genome analyses of the three isolates demonstrated that they were closely related but distinct strains of a novel virus, designated Tarumizu tick virus (TarTV), which has a genome of 12 double-stranded RNA segments. The development of the virus-induced cytopathic effects on BHK cells significantly varied according to virus strains. Ten out of 12 segments of TarTV appeared to encode putative orthologs or functional equivalents of viral proteins of Colorado tick fever virus (CTFV) and Eyach virus, suggesting that TarTV is the third member of the genus Coltivirus in the family Reoviridae. This was supported by the facts that the 5′- and 3′-terminal consensus sequences of coltivirus genomes were found also in TarTV genome, and segment 9 of TarTV had sequence and structural features that may mediate a stop codon read-through as observed in that of CTFV. However, segment 7 and 10 of TarTV had no significant sequence similarities to any other proteins of known coltiviruses. Electron microscopic analysis demonstrated that TarTV particle had a non-enveloped bilayer icosahedral structure, and viral inclusion bodies were formed in infected cells. TarTV could infect and replicate in several mammalian cell lines tested, but show no clinical symptoms in intracerebrally inoculated mice. Taken together, our findings provide new insights into genetic diversity and evolution of the genus Coltivirus.

Original languageEnglish
Pages (from-to)131-140
Number of pages10
JournalVirus Research
Volume242
DOIs
Publication statusPublished - Oct 15 2017
Externally publishedYes

Fingerprint

Coltivirus
Ticks
Japan
Viruses
Colorado tick fever virus
Genome
Viral Inclusion Bodies
Reoviridae
Ixodidae
Molecular Evolution
Double-Stranded RNA
Terminator Codon

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Isolation and characterization of Tarumizu tick virus : A new coltivirus from Haemaphysalis flava ticks in Japan. / Fujita, Ryosuke; Ejiri, Hiroko; Lim, Chang Kweng; Noda, Shinichi; Yamauchi, Takeo; Watanabe, Mamoru; Kobayashi, Daisuke; Takayama-Ito, Mutsuyo; Murota, Katsunori; Posadas-Herrera, Guillermo; Minami, Shohei; Kuwata, Ryusei; Yamaguchi, Yukie; Horiya, Madoka; Katayama, Yukie; Shimoda, Hiroshi; Saijo, Masayuki; Maeda, Ken; Mizutani, Tetsuya; Isawa, Haruhiko; Sawabe, Kyoko.

In: Virus Research, Vol. 242, 15.10.2017, p. 131-140.

Research output: Contribution to journalArticle

Fujita, R, Ejiri, H, Lim, CK, Noda, S, Yamauchi, T, Watanabe, M, Kobayashi, D, Takayama-Ito, M, Murota, K, Posadas-Herrera, G, Minami, S, Kuwata, R, Yamaguchi, Y, Horiya, M, Katayama, Y, Shimoda, H, Saijo, M, Maeda, K, Mizutani, T, Isawa, H & Sawabe, K 2017, 'Isolation and characterization of Tarumizu tick virus: A new coltivirus from Haemaphysalis flava ticks in Japan', Virus Research, vol. 242, pp. 131-140. https://doi.org/10.1016/j.virusres.2017.09.017
Fujita, Ryosuke ; Ejiri, Hiroko ; Lim, Chang Kweng ; Noda, Shinichi ; Yamauchi, Takeo ; Watanabe, Mamoru ; Kobayashi, Daisuke ; Takayama-Ito, Mutsuyo ; Murota, Katsunori ; Posadas-Herrera, Guillermo ; Minami, Shohei ; Kuwata, Ryusei ; Yamaguchi, Yukie ; Horiya, Madoka ; Katayama, Yukie ; Shimoda, Hiroshi ; Saijo, Masayuki ; Maeda, Ken ; Mizutani, Tetsuya ; Isawa, Haruhiko ; Sawabe, Kyoko. / Isolation and characterization of Tarumizu tick virus : A new coltivirus from Haemaphysalis flava ticks in Japan. In: Virus Research. 2017 ; Vol. 242. pp. 131-140.
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abstract = "During the course of tick-borne virus surveillance in Japan, three independent isolates of probably the same virus were obtained from three geographically distant populations of the hard tick Haemaphysalis flava. Genome analyses of the three isolates demonstrated that they were closely related but distinct strains of a novel virus, designated Tarumizu tick virus (TarTV), which has a genome of 12 double-stranded RNA segments. The development of the virus-induced cytopathic effects on BHK cells significantly varied according to virus strains. Ten out of 12 segments of TarTV appeared to encode putative orthologs or functional equivalents of viral proteins of Colorado tick fever virus (CTFV) and Eyach virus, suggesting that TarTV is the third member of the genus Coltivirus in the family Reoviridae. This was supported by the facts that the 5′- and 3′-terminal consensus sequences of coltivirus genomes were found also in TarTV genome, and segment 9 of TarTV had sequence and structural features that may mediate a stop codon read-through as observed in that of CTFV. However, segment 7 and 10 of TarTV had no significant sequence similarities to any other proteins of known coltiviruses. Electron microscopic analysis demonstrated that TarTV particle had a non-enveloped bilayer icosahedral structure, and viral inclusion bodies were formed in infected cells. TarTV could infect and replicate in several mammalian cell lines tested, but show no clinical symptoms in intracerebrally inoculated mice. Taken together, our findings provide new insights into genetic diversity and evolution of the genus Coltivirus.",
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AU - Noda, Shinichi

AU - Yamauchi, Takeo

AU - Watanabe, Mamoru

AU - Kobayashi, Daisuke

AU - Takayama-Ito, Mutsuyo

AU - Murota, Katsunori

AU - Posadas-Herrera, Guillermo

AU - Minami, Shohei

AU - Kuwata, Ryusei

AU - Yamaguchi, Yukie

AU - Horiya, Madoka

AU - Katayama, Yukie

AU - Shimoda, Hiroshi

AU - Saijo, Masayuki

AU - Maeda, Ken

AU - Mizutani, Tetsuya

AU - Isawa, Haruhiko

AU - Sawabe, Kyoko

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