Isolation and characterization of tetranucleotide microsatellite markers for Pinus merkusii

D. V. Thao, A. Y.P.B.C. Widyatmoko, L. Guan, Eiji Gotoh, Atsushi Watanabe, S. Shiraishi

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1 Citation (Scopus)

Abstract

Pinus merkusii is an important tree native to Southeast Asia. Thirteen tetranucleotide microsatellite makers were developed from this species using a dual-suppression polymerase chain reaction technique. Of these markers, nine loci were polymorphic. The mean number of alleles per locus was 3. 18 (range 2-5) and mean expected heterozygosities were 0. 525 (range 0. 384-0. 712). These microsatellite markers will be available to assess the genetic diversity, mating patterns and population structure which will facilitate conservation and management strategies.

Original languageEnglish
Pages (from-to)433-436
Number of pages4
JournalConservation Genetics Resources
Volume5
Issue number2
DOIs
Publication statusPublished - May 3 2013

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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