Isolation and characterization of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation.

M. Ohtsubo, R. Kai, N. Furuno, Takeshi Sekiguchi, M. Sekiguchi, H. Hayashida, K. Kuma, T. Miyata, S. Fukushige, T. Murotsu

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

The human RCC1 gene was cloned after DNA-mediated gene transfer into the tsBN2 cell line, which shows premature chromosome condensation at nonpermissive temperatures (39.5-40 degrees C). This gene codes for a 2.5-kb poly(A)+ RNA that is well conserved in hamsters and humans. We isolated 15 cDNA clones from the Okayama-Berg human cDNA library, and found two that can complement the tsBN2 mutation with an efficiency comparable to that of the genomic DNA clone. The base sequences of these two active cDNA clones differ at the 5' proximal end, yet both have a common open reading frame, encoding a protein of 421 amino acids with a calculated molecular weight of 44,847 and with seven homologous repeated domains of about 60 amino acids. This human RCC1 gene was located to human chromosome 1 using sorted chromosomal fractions.

Original languageEnglish
Pages (from-to)585-593
Number of pages9
JournalGenes & development
Volume1
Issue number6
DOIs
Publication statusPublished - Jan 1 1987

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cdc Genes
Complementary DNA
Chromosomes
Clone Cells
Genes
Amino Acids
Chromosomes, Human, Pair 1
DNA
Human Chromosomes
Gene Library
Cricetinae
Open Reading Frames
Molecular Weight
Cell Line
Messenger RNA
Mutation
Temperature
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Cite this

Isolation and characterization of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation. / Ohtsubo, M.; Kai, R.; Furuno, N.; Sekiguchi, Takeshi; Sekiguchi, M.; Hayashida, H.; Kuma, K.; Miyata, T.; Fukushige, S.; Murotsu, T.

In: Genes & development, Vol. 1, No. 6, 01.01.1987, p. 585-593.

Research output: Contribution to journalArticle

Ohtsubo, M, Kai, R, Furuno, N, Sekiguchi, T, Sekiguchi, M, Hayashida, H, Kuma, K, Miyata, T, Fukushige, S & Murotsu, T 1987, 'Isolation and characterization of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation.', Genes & development, vol. 1, no. 6, pp. 585-593. https://doi.org/10.1101/gad.1.6.585
Ohtsubo, M. ; Kai, R. ; Furuno, N. ; Sekiguchi, Takeshi ; Sekiguchi, M. ; Hayashida, H. ; Kuma, K. ; Miyata, T. ; Fukushige, S. ; Murotsu, T. / Isolation and characterization of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation. In: Genes & development. 1987 ; Vol. 1, No. 6. pp. 585-593.
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