Isolation and characterization of the dnaKJ operon from Actinobacillus actinomycetemcomitans

Akihiro Yoshida, Yoshio Nakano, Yoshihisa Yamashita, Hao Yu, Masamichi Ohishi, Toshihiko Koga

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The dnaKJ operon of Actinobacillus actinomycetemcomitans Y4 was cloned by the DNA-probing method using synthetic oligonucleotides designed on the basis of two conserved regions in DnaK/hsp70 proteins and sequenced by inverse PCR. The sequenced region was shown to contain two open reading frames coding for proteins analogous to DnaK and DnaJ.

Original languageEnglish
Pages (from-to)93-98
Number of pages6
JournalMitochondrial DNA
Volume8
Issue number1-2
DOIs
Publication statusPublished - Dec 1 1997

Fingerprint

Aggregatibacter actinomycetemcomitans
Operon
Oligonucleotides
Open Reading Frames
Proteins
Polymerase Chain Reaction
DNA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Isolation and characterization of the dnaKJ operon from Actinobacillus actinomycetemcomitans. / Yoshida, Akihiro; Nakano, Yoshio; Yamashita, Yoshihisa; Yu, Hao; Ohishi, Masamichi; Koga, Toshihiko.

In: Mitochondrial DNA, Vol. 8, No. 1-2, 01.12.1997, p. 93-98.

Research output: Contribution to journalArticle

Yoshida, Akihiro ; Nakano, Yoshio ; Yamashita, Yoshihisa ; Yu, Hao ; Ohishi, Masamichi ; Koga, Toshihiko. / Isolation and characterization of the dnaKJ operon from Actinobacillus actinomycetemcomitans. In: Mitochondrial DNA. 1997 ; Vol. 8, No. 1-2. pp. 93-98.
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