Isolation and characterization of the human ornithinc transcarbamylase gene: Structure of the 5′-end region

Akira Hata, Teruhisa Tsuzuki, Kazunori Shimada, Masaki Takiguchi, Masataka Mori, Ichiro Matsuda

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Abstract

We isolated over 20 phage clones carrying the ornithine transcarbamylase (OTC) [EC 2.1.3.3] gene, from two independently constructed human genomic DNA libraries, using as probes either a rat OTC eDNA or several nuclear DNA fragments derived from some of these isolated clones. These clones, classified into 10 different groups, overlapped and spanned a region of more than 85 kilobase pairs of the human genomic DNA. Restriction mapping and Southern blot analyses demonstrated that one of the clones covers the 5′-end region of the OTC gene. We sequenced the 5′-end region of the OTC gene and found that it covered 665 base pairs of the 5′-flanking region, the complete first exon and a part of the first intron (150 base pairs). In the 5′-flanking region, there were two pairs of putative CAAT and TATA boxes and one enhancer core-like sequence, GTGGAAAG. The first exon contained a coding region for most of the OTC presequence, i.e. 26 out of 32 amino acid residues of the presequence, including the initiation methionine.

Original languageEnglish
Pages (from-to)717-725
Number of pages9
JournalJournal of Biochemistry
Volume100
Issue number3
DOIs
Publication statusPublished - Jan 1 1986

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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