Isolation and characterization of two β-d-glucosidases from bifidobacterium breve 203

Kenji Sakai, Takashi Tachiki, Hidehiko Kumagai, Tatsurokuro Tochkura

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Abstract

Two β-D-glucosidases were purified to homogeneity from Bifidobacterium breve 203: One (β-D-glucosidase I; molecular weight, 96,000) showed reactivity toward β-nitrophenyl O-NP) β-D-fucoside, 74% of that to p-NP β-D-glucoside, and the other β-D-glucosidase II; molecular weight, 450,000) did not. They also differed in their thermal and pH stabilities. Laminaribiose, cellobiose and gentiobiose were hydrolyzed by β-D-glucosidase I, with 53%, 34% and 3% of the reactivity inthe case ofp-NP β-D-glucoside, and by β -D-glucosidase II, with 53%, 6% and 107% of the reactivity. The reaction of β-D-glucosidase I with p-NP β-D-fucoside was enhanced by the addition of glucose and other monosaccharides to the reaction mixture, whereas that with p-NP β-D-glucoside was notaffected. The activity of β-D-glucosidase II with p-NP β-D-glucoside was inhibited by glucose.

Original languageEnglish
Pages (from-to)2287-2293
Number of pages7
JournalAgricultural and Biological Chemistry
Volume50
Issue number9
DOIs
Publication statusPublished - Sep 1986
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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