Isolation and expression of FIP-2 in wounded pulp of the rat

M. Oyama, F. Myokai, T. Ohira, N. Shiomi, K. Yamashiro, H. Arai, Fusanori Nishimura, S. Takashiba

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Pulpal wound healing followed by cavity preparation may involve reactionary or reparative dentinogenesis in relation to the cavity position; however, little is known about the molecular responses. We aimed to isolate and analyze genes induced or suppressed in the wounded pulp to identify molecular processes involved in the pulp responses to injury. Twenty-three cDNAs were isolated by cDNA subtraction between healthy and wounded pulp of rats. By library screening, we identified rat 14.7K-interacting protein (rFIP)-2A and B genes homologous to human FIP-2, being involved in regulating membrane trafficking and cellular morphogenesis. RT-PCR analysis showed induction for only rFIP-2B in the wounded pulp. In situ hybridization analysis revealed that both rFIP-2s were expressed strongly in condensing mesenchymal cells of the palatal process and surrounding Meckel's cartilage, but not in intramembranous chondrogenic cells. Thus, up-regulated rFIP-2B expression may play a role in regulating membrane trafficking or cellular morphogenesis of these embryonic and wounded pulpal cells.

Original languageEnglish
Pages (from-to)842-847
Number of pages6
JournalJournal of Dental Research
Volume84
Issue number9
DOIs
Publication statusPublished - Sep 1 2005
Externally publishedYes

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Morphogenesis
Dentinogenesis
Complementary DNA
Membranes
Wound Healing
Genes
Cartilage
In Situ Hybridization
Polymerase Chain Reaction
Wounds and Injuries
rat FIP-2A protein

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Oyama, M., Myokai, F., Ohira, T., Shiomi, N., Yamashiro, K., Arai, H., ... Takashiba, S. (2005). Isolation and expression of FIP-2 in wounded pulp of the rat. Journal of Dental Research, 84(9), 842-847. https://doi.org/10.1177/154405910508400912

Isolation and expression of FIP-2 in wounded pulp of the rat. / Oyama, M.; Myokai, F.; Ohira, T.; Shiomi, N.; Yamashiro, K.; Arai, H.; Nishimura, Fusanori; Takashiba, S.

In: Journal of Dental Research, Vol. 84, No. 9, 01.09.2005, p. 842-847.

Research output: Contribution to journalArticle

Oyama, M, Myokai, F, Ohira, T, Shiomi, N, Yamashiro, K, Arai, H, Nishimura, F & Takashiba, S 2005, 'Isolation and expression of FIP-2 in wounded pulp of the rat', Journal of Dental Research, vol. 84, no. 9, pp. 842-847. https://doi.org/10.1177/154405910508400912
Oyama M, Myokai F, Ohira T, Shiomi N, Yamashiro K, Arai H et al. Isolation and expression of FIP-2 in wounded pulp of the rat. Journal of Dental Research. 2005 Sep 1;84(9):842-847. https://doi.org/10.1177/154405910508400912
Oyama, M. ; Myokai, F. ; Ohira, T. ; Shiomi, N. ; Yamashiro, K. ; Arai, H. ; Nishimura, Fusanori ; Takashiba, S. / Isolation and expression of FIP-2 in wounded pulp of the rat. In: Journal of Dental Research. 2005 ; Vol. 84, No. 9. pp. 842-847.
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