TY - JOUR
T1 - Isolation and partial characterization of the Chlamydomonas reinhardtii γ-glutamyl kinase
AU - Miyoshi, Keita
AU - Matsuura, Hideyuki
AU - Kumon, Takao
AU - Matsuda, Keita
AU - Yoshikawa, Tomoaki
AU - Bamba, Takeshi
AU - Harada, Kazuo
AU - Hirata, Kazumasa
PY - 2011/7/1
Y1 - 2011/7/1
N2 - We isolated a Chlamydomonas gene encoding putative γ-glutamyl kinase (GK), an enzyme that catalyzes the first step of proline biosynthesis. Using an Escherichia coli auxotroph and a purified recombinant protein, we show that Chlamydomonas GK is a functional GK. The sensitivity of this kinase to feedback inhibition by proline was lower than in that of microbial GKs previously reported.
AB - We isolated a Chlamydomonas gene encoding putative γ-glutamyl kinase (GK), an enzyme that catalyzes the first step of proline biosynthesis. Using an Escherichia coli auxotroph and a purified recombinant protein, we show that Chlamydomonas GK is a functional GK. The sensitivity of this kinase to feedback inhibition by proline was lower than in that of microbial GKs previously reported.
UR - http://www.scopus.com/inward/record.url?scp=79960249184&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79960249184&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2011.02.012
DO - 10.1016/j.jbiosc.2011.02.012
M3 - Article
C2 - 21444242
AN - SCOPUS:79960249184
VL - 112
SP - 75
EP - 78
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 1
ER -