Isolation and partial characterization of the Chlamydomonas reinhardtii γ-glutamyl kinase

Keita Miyoshi, Hideyuki Matsuura, Takao Kumon, Keita Matsuda, Tomoaki Yoshikawa, Takeshi Bamba, Kazuo Harada, Kazumasa Hirata

Research output: Contribution to journalArticlepeer-review

Abstract

We isolated a Chlamydomonas gene encoding putative γ-glutamyl kinase (GK), an enzyme that catalyzes the first step of proline biosynthesis. Using an Escherichia coli auxotroph and a purified recombinant protein, we show that Chlamydomonas GK is a functional GK. The sensitivity of this kinase to feedback inhibition by proline was lower than in that of microbial GKs previously reported.

Original languageEnglish
Pages (from-to)75-78
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume112
Issue number1
DOIs
Publication statusPublished - Jul 1 2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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