Abstract
We isolated a Chlamydomonas gene encoding putative γ-glutamyl kinase (GK), an enzyme that catalyzes the first step of proline biosynthesis. Using an Escherichia coli auxotroph and a purified recombinant protein, we show that Chlamydomonas GK is a functional GK. The sensitivity of this kinase to feedback inhibition by proline was lower than in that of microbial GKs previously reported.
| Original language | English |
|---|---|
| Pages (from-to) | 75-78 |
| Number of pages | 4 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 112 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jul 1 2011 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Cite this
Isolation and partial characterization of the Chlamydomonas reinhardtii γ-glutamyl kinase. / Miyoshi, Keita; Matsuura, Hideyuki; Kumon, Takao; Matsuda, Keita; Yoshikawa, Tomoaki; Bamba, Takeshi; Harada, Kazuo; Hirata, Kazumasa.
In: Journal of Bioscience and Bioengineering, Vol. 112, No. 1, 01.07.2011, p. 75-78.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and partial characterization of the Chlamydomonas reinhardtii γ-glutamyl kinase
AU - Miyoshi, Keita
AU - Matsuura, Hideyuki
AU - Kumon, Takao
AU - Matsuda, Keita
AU - Yoshikawa, Tomoaki
AU - Bamba, Takeshi
AU - Harada, Kazuo
AU - Hirata, Kazumasa
PY - 2011/7/1
Y1 - 2011/7/1
N2 - We isolated a Chlamydomonas gene encoding putative γ-glutamyl kinase (GK), an enzyme that catalyzes the first step of proline biosynthesis. Using an Escherichia coli auxotroph and a purified recombinant protein, we show that Chlamydomonas GK is a functional GK. The sensitivity of this kinase to feedback inhibition by proline was lower than in that of microbial GKs previously reported.
AB - We isolated a Chlamydomonas gene encoding putative γ-glutamyl kinase (GK), an enzyme that catalyzes the first step of proline biosynthesis. Using an Escherichia coli auxotroph and a purified recombinant protein, we show that Chlamydomonas GK is a functional GK. The sensitivity of this kinase to feedback inhibition by proline was lower than in that of microbial GKs previously reported.
UR - http://www.scopus.com/inward/record.url?scp=79960249184&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79960249184&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2011.02.012
DO - 10.1016/j.jbiosc.2011.02.012
M3 - Article
C2 - 21444242
AN - SCOPUS:79960249184
VL - 112
SP - 75
EP - 78
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 1
ER -