Isolation and partial characterization of the Chlamydomonas reinhardtii γ-glutamyl kinase

Keita Miyoshi, Hideyuki Matsuura, Takao Kumon, Keita Matsuda, Tomoaki Yoshikawa, Takeshi Bamba, Kazuo Harada, Kazumasa Hirata

Research output: Contribution to journalArticle

Abstract

We isolated a Chlamydomonas gene encoding putative γ-glutamyl kinase (GK), an enzyme that catalyzes the first step of proline biosynthesis. Using an Escherichia coli auxotroph and a purified recombinant protein, we show that Chlamydomonas GK is a functional GK. The sensitivity of this kinase to feedback inhibition by proline was lower than in that of microbial GKs previously reported.

Original languageEnglish
Pages (from-to)75-78
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume112
Issue number1
DOIs
Publication statusPublished - Jul 1 2011
Externally publishedYes

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Chlamydomonas reinhardtii
Recombinant proteins
Gene encoding
Biosynthesis
Escherichia coli
Phosphotransferases
Enzymes
Chlamydomonas
Feedback
Proline
Recombinant Proteins
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Isolation and partial characterization of the Chlamydomonas reinhardtii γ-glutamyl kinase. / Miyoshi, Keita; Matsuura, Hideyuki; Kumon, Takao; Matsuda, Keita; Yoshikawa, Tomoaki; Bamba, Takeshi; Harada, Kazuo; Hirata, Kazumasa.

In: Journal of Bioscience and Bioengineering, Vol. 112, No. 1, 01.07.2011, p. 75-78.

Research output: Contribution to journalArticle

Miyoshi, K, Matsuura, H, Kumon, T, Matsuda, K, Yoshikawa, T, Bamba, T, Harada, K & Hirata, K 2011, 'Isolation and partial characterization of the Chlamydomonas reinhardtii γ-glutamyl kinase', Journal of Bioscience and Bioengineering, vol. 112, no. 1, pp. 75-78. https://doi.org/10.1016/j.jbiosc.2011.02.012
Miyoshi, Keita ; Matsuura, Hideyuki ; Kumon, Takao ; Matsuda, Keita ; Yoshikawa, Tomoaki ; Bamba, Takeshi ; Harada, Kazuo ; Hirata, Kazumasa. / Isolation and partial characterization of the Chlamydomonas reinhardtii γ-glutamyl kinase. In: Journal of Bioscience and Bioengineering. 2011 ; Vol. 112, No. 1. pp. 75-78.
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