Isolation and partial characterization of the Chlamydomonas reinhardtii γ-glutamyl kinase

Keita Miyoshi; Hideyuki Matsuura; Takao Kumon; Keita Matsuda; Tomoaki Yoshikawa; Takeshi Bamba; Kazuo Harada; Kazumasa Hirata

doi:10.1016/j.jbiosc.2011.02.012

Isolation and partial characterization of the Chlamydomonas reinhardtii γ-glutamyl kinase

Keita Miyoshi, Hideyuki Matsuura, Takao Kumon, Keita Matsuda, Tomoaki Yoshikawa, Takeshi Bamba, Kazuo Harada, Kazumasa Hirata

Research output: Contribution to journal › Article › peer-review

Abstract

We isolated a Chlamydomonas gene encoding putative γ-glutamyl kinase (GK), an enzyme that catalyzes the first step of proline biosynthesis. Using an Escherichia coli auxotroph and a purified recombinant protein, we show that Chlamydomonas GK is a functional GK. The sensitivity of this kinase to feedback inhibition by proline was lower than in that of microbial GKs previously reported.

Original language	English
Pages (from-to)	75-78
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	112
Issue number	1
DOIs	https://doi.org/10.1016/j.jbiosc.2011.02.012
Publication status	Published - Jul 1 2011
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.jbiosc.2011.02.012

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Isolation and partial characterization of the Chlamydomonas reinhardtii γ-glutamyl kinase. / Miyoshi, Keita; Matsuura, Hideyuki; Kumon, Takao; Matsuda, Keita; Yoshikawa, Tomoaki; Bamba, Takeshi; Harada, Kazuo; Hirata, Kazumasa.

In: Journal of Bioscience and Bioengineering, Vol. 112, No. 1, 01.07.2011, p. 75-78.

Research output: Contribution to journal › Article › peer-review

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AU - Yoshikawa, Tomoaki

AU - Bamba, Takeshi

AU - Harada, Kazuo

AU - Hirata, Kazumasa

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