Isolation and properties of membrane-bound cytochrome c-552 from photosynthetic bacterium Chromatium vinosum

Michio Doi, Ken Ichiro Takamiya, Mitsuo Nishimura

Research output: Contribution to journalArticle

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Abstract

A membrane-bound cytochrome c-552 was isolated and purified from the photosynthetic bacterium Chromatium vinosum by treatment with sodium cholate, sodium deoxycholate and bacterial alkaline protease followed by gel filtration. The purified cytochrome c-552, which may have been modified by the protease treatment, was electrophoretically homogeneous. Its minimal molecular weight was estimated to be 19 and 20 kdaltons, respectively by SDS polyacrylamide gel electrophoresis and by gel filtration on Sephadex G-100. Cytochrome c-552 showed the absorption maxima at 419, 523 and 552 nm in the reduced form. Reduced-minus-oxidized difference millimolar absorption coefficient was 10.6 for the wavelength pair, 552 minus 540 nm. The midpoint potential at pH 8.0 was -130 mV. The polarity in the amino acid composition of cytochrome c-552 was 40.1% and reflected its hydrophobicity. The solubilized cytochrome c-552 was shown to be a different entity from the soluble flavocytochrome c-552 in several respects.

Original languageEnglish
Pages (from-to)49-60
Number of pages12
JournalPhotosynthesis Research
Volume4
Issue number1
DOIs
Publication statusPublished - Jan 1983

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Allochromatium vinosum
Chromatium
photosynthetic bacteria
cytochrome c
Cytochromes c
Bacteria
Membranes
Gel Chromatography
proteinases
Gels
Sodium Cholate
gels
deoxycholic acid
Deoxycholic Acid
Hydrophobicity
hydrophobicity
Electrophoresis
Hydrophobic and Hydrophilic Interactions
amino acid composition
polyacrylamide gel electrophoresis

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Isolation and properties of membrane-bound cytochrome c-552 from photosynthetic bacterium Chromatium vinosum. / Doi, Michio; Takamiya, Ken Ichiro; Nishimura, Mitsuo.

In: Photosynthesis Research, Vol. 4, No. 1, 01.1983, p. 49-60.

Research output: Contribution to journalArticle

Doi, Michio ; Takamiya, Ken Ichiro ; Nishimura, Mitsuo. / Isolation and properties of membrane-bound cytochrome c-552 from photosynthetic bacterium Chromatium vinosum. In: Photosynthesis Research. 1983 ; Vol. 4, No. 1. pp. 49-60.
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