Isolation and sequence determination of human brain natriuretic peptide in human atrium

Yoshikazu Kambayashi; Kazuwa Nakao; Masashi Mukoyama; Yoshihiko Saito; Yoshihiro Ogawa; Shozo Shiono; Ken Inouye; Nobuo Yoshida; Hiroo Imura

doi:10.1016/0014-5793(90)80043-I

Isolation and sequence determination of human brain natriuretic peptide in human atrium

Yoshikazu Kambayashi, Kazuwa Nakao, Masashi Mukoyama, Yoshihiko Saito, Yoshihiro Ogawa, Shozo Shiono, Ken Inouye, Nobuo Yoshida, Hiroo Imura

Internal Medicine

Research output: Contribution to journal › Article

214 Citations (Scopus)

Abstract

We isolated human brain natriuretic peptide (human BNP) from the human atrium. Sequence analysis has revealed that it is a 32-amino-acid peptide with the sequence S-P-K-M-V-Q-G-S-G-C-F-G-R-K-M-D-R-I-S-S-S-S-G-L-G-C-K-V-L-R-R-H, which is identical to the C-terminal sequence (77-108) of the human BNP precursor deduced from the cDNA sequence. The sequence of human BNP (77-108) is preceded by Pro⁷⁵-Arg⁷⁶ in the human BNP precursor, which is the same processing signal as Pro⁹⁷-Arg⁹⁸ of the precursor of atrial natriuretic peptide (ANP). The processing of the BNP precursor occurs in the cardiocyte, although that of the ANP precursor in the cardiocyte is unclear at present.

Original language	English
Pages (from-to)	341-345
Number of pages	5
Journal	FEBS Letters
Volume	259
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(90)80043-I
Publication status	Published - Jan 1 1990

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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Kambayashi, Y., Nakao, K., Mukoyama, M., Saito, Y., Ogawa, Y., Shiono, S., Inouye, K., Yoshida, N., & Imura, H. (1990). Isolation and sequence determination of human brain natriuretic peptide in human atrium. FEBS Letters, 259(2), 341-345. https://doi.org/10.1016/0014-5793(90)80043-I

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abstract = "We isolated human brain natriuretic peptide (human BNP) from the human atrium. Sequence analysis has revealed that it is a 32-amino-acid peptide with the sequence S-P-K-M-V-Q-G-S-G-C-F-G-R-K-M-D-R-I-S-S-S-S-G-L-G-C-K-V-L-R-R-H, which is identical to the C-terminal sequence (77-108) of the human BNP precursor deduced from the cDNA sequence. The sequence of human BNP (77-108) is preceded by Pro75-Arg76 in the human BNP precursor, which is the same processing signal as Pro97-Arg98 of the precursor of atrial natriuretic peptide (ANP). The processing of the BNP precursor occurs in the cardiocyte, although that of the ANP precursor in the cardiocyte is unclear at present.",

author = "Yoshikazu Kambayashi and Kazuwa Nakao and Masashi Mukoyama and Yoshihiko Saito and Yoshihiro Ogawa and Shozo Shiono and Ken Inouye and Nobuo Yoshida and Hiroo Imura",

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AU - Kambayashi, Yoshikazu

AU - Nakao, Kazuwa

AU - Mukoyama, Masashi

AU - Saito, Yoshihiko

AU - Ogawa, Yoshihiro

AU - Shiono, Shozo

AU - Inouye, Ken

AU - Yoshida, Nobuo

AU - Imura, Hiroo

PY - 1990/1/1

Y1 - 1990/1/1

N2 - We isolated human brain natriuretic peptide (human BNP) from the human atrium. Sequence analysis has revealed that it is a 32-amino-acid peptide with the sequence S-P-K-M-V-Q-G-S-G-C-F-G-R-K-M-D-R-I-S-S-S-S-G-L-G-C-K-V-L-R-R-H, which is identical to the C-terminal sequence (77-108) of the human BNP precursor deduced from the cDNA sequence. The sequence of human BNP (77-108) is preceded by Pro75-Arg76 in the human BNP precursor, which is the same processing signal as Pro97-Arg98 of the precursor of atrial natriuretic peptide (ANP). The processing of the BNP precursor occurs in the cardiocyte, although that of the ANP precursor in the cardiocyte is unclear at present.

AB - We isolated human brain natriuretic peptide (human BNP) from the human atrium. Sequence analysis has revealed that it is a 32-amino-acid peptide with the sequence S-P-K-M-V-Q-G-S-G-C-F-G-R-K-M-D-R-I-S-S-S-S-G-L-G-C-K-V-L-R-R-H, which is identical to the C-terminal sequence (77-108) of the human BNP precursor deduced from the cDNA sequence. The sequence of human BNP (77-108) is preceded by Pro75-Arg76 in the human BNP precursor, which is the same processing signal as Pro97-Arg98 of the precursor of atrial natriuretic peptide (ANP). The processing of the BNP precursor occurs in the cardiocyte, although that of the ANP precursor in the cardiocyte is unclear at present.

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