Isolation and sequence determination of human brain natriuretic peptide in human atrium

Yoshikazu Kambayashi, Kazuwa Nakao, Masashi Mukoyama, Yoshihiko Saito, Yoshihiro Ogawa, Shozo Shiono, Ken Inouye, Nobuo Yoshida, Hiroo Imura

Research output: Contribution to journalArticle

211 Citations (Scopus)

Abstract

We isolated human brain natriuretic peptide (human BNP) from the human atrium. Sequence analysis has revealed that it is a 32-amino-acid peptide with the sequence S-P-K-M-V-Q-G-S-G-C-F-G-R-K-M-D-R-I-S-S-S-S-G-L-G-C-K-V-L-R-R-H, which is identical to the C-terminal sequence (77-108) of the human BNP precursor deduced from the cDNA sequence. The sequence of human BNP (77-108) is preceded by Pro75-Arg76 in the human BNP precursor, which is the same processing signal as Pro97-Arg98 of the precursor of atrial natriuretic peptide (ANP). The processing of the BNP precursor occurs in the cardiocyte, although that of the ANP precursor in the cardiocyte is unclear at present.

Original languageEnglish
Pages (from-to)341-345
Number of pages5
JournalFEBS Letters
Volume259
Issue number2
DOIs
Publication statusPublished - Jan 1 1990
Externally publishedYes

Fingerprint

Brain Natriuretic Peptide
Sequence Analysis
Atrial Natriuretic Factor
Signal processing
Complementary DNA
Amino Acids
Peptides
Processing

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Kambayashi, Y., Nakao, K., Mukoyama, M., Saito, Y., Ogawa, Y., Shiono, S., ... Imura, H. (1990). Isolation and sequence determination of human brain natriuretic peptide in human atrium. FEBS Letters, 259(2), 341-345. https://doi.org/10.1016/0014-5793(90)80043-I

Isolation and sequence determination of human brain natriuretic peptide in human atrium. / Kambayashi, Yoshikazu; Nakao, Kazuwa; Mukoyama, Masashi; Saito, Yoshihiko; Ogawa, Yoshihiro; Shiono, Shozo; Inouye, Ken; Yoshida, Nobuo; Imura, Hiroo.

In: FEBS Letters, Vol. 259, No. 2, 01.01.1990, p. 341-345.

Research output: Contribution to journalArticle

Kambayashi, Y, Nakao, K, Mukoyama, M, Saito, Y, Ogawa, Y, Shiono, S, Inouye, K, Yoshida, N & Imura, H 1990, 'Isolation and sequence determination of human brain natriuretic peptide in human atrium', FEBS Letters, vol. 259, no. 2, pp. 341-345. https://doi.org/10.1016/0014-5793(90)80043-I
Kambayashi, Yoshikazu ; Nakao, Kazuwa ; Mukoyama, Masashi ; Saito, Yoshihiko ; Ogawa, Yoshihiro ; Shiono, Shozo ; Inouye, Ken ; Yoshida, Nobuo ; Imura, Hiroo. / Isolation and sequence determination of human brain natriuretic peptide in human atrium. In: FEBS Letters. 1990 ; Vol. 259, No. 2. pp. 341-345.
@article{3f977ad4fe984b9194010e9c2a6b162b,
title = "Isolation and sequence determination of human brain natriuretic peptide in human atrium",
abstract = "We isolated human brain natriuretic peptide (human BNP) from the human atrium. Sequence analysis has revealed that it is a 32-amino-acid peptide with the sequence S-P-K-M-V-Q-G-S-G-C-F-G-R-K-M-D-R-I-S-S-S-S-G-L-G-C-K-V-L-R-R-H, which is identical to the C-terminal sequence (77-108) of the human BNP precursor deduced from the cDNA sequence. The sequence of human BNP (77-108) is preceded by Pro75-Arg76 in the human BNP precursor, which is the same processing signal as Pro97-Arg98 of the precursor of atrial natriuretic peptide (ANP). The processing of the BNP precursor occurs in the cardiocyte, although that of the ANP precursor in the cardiocyte is unclear at present.",
author = "Yoshikazu Kambayashi and Kazuwa Nakao and Masashi Mukoyama and Yoshihiko Saito and Yoshihiro Ogawa and Shozo Shiono and Ken Inouye and Nobuo Yoshida and Hiroo Imura",
year = "1990",
month = "1",
day = "1",
doi = "10.1016/0014-5793(90)80043-I",
language = "English",
volume = "259",
pages = "341--345",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Isolation and sequence determination of human brain natriuretic peptide in human atrium

AU - Kambayashi, Yoshikazu

AU - Nakao, Kazuwa

AU - Mukoyama, Masashi

AU - Saito, Yoshihiko

AU - Ogawa, Yoshihiro

AU - Shiono, Shozo

AU - Inouye, Ken

AU - Yoshida, Nobuo

AU - Imura, Hiroo

PY - 1990/1/1

Y1 - 1990/1/1

N2 - We isolated human brain natriuretic peptide (human BNP) from the human atrium. Sequence analysis has revealed that it is a 32-amino-acid peptide with the sequence S-P-K-M-V-Q-G-S-G-C-F-G-R-K-M-D-R-I-S-S-S-S-G-L-G-C-K-V-L-R-R-H, which is identical to the C-terminal sequence (77-108) of the human BNP precursor deduced from the cDNA sequence. The sequence of human BNP (77-108) is preceded by Pro75-Arg76 in the human BNP precursor, which is the same processing signal as Pro97-Arg98 of the precursor of atrial natriuretic peptide (ANP). The processing of the BNP precursor occurs in the cardiocyte, although that of the ANP precursor in the cardiocyte is unclear at present.

AB - We isolated human brain natriuretic peptide (human BNP) from the human atrium. Sequence analysis has revealed that it is a 32-amino-acid peptide with the sequence S-P-K-M-V-Q-G-S-G-C-F-G-R-K-M-D-R-I-S-S-S-S-G-L-G-C-K-V-L-R-R-H, which is identical to the C-terminal sequence (77-108) of the human BNP precursor deduced from the cDNA sequence. The sequence of human BNP (77-108) is preceded by Pro75-Arg76 in the human BNP precursor, which is the same processing signal as Pro97-Arg98 of the precursor of atrial natriuretic peptide (ANP). The processing of the BNP precursor occurs in the cardiocyte, although that of the ANP precursor in the cardiocyte is unclear at present.

UR - http://www.scopus.com/inward/record.url?scp=0025015089&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025015089&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(90)80043-I

DO - 10.1016/0014-5793(90)80043-I

M3 - Article

C2 - 2136732

AN - SCOPUS:0025015089

VL - 259

SP - 341

EP - 345

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -