Abstract
We isolated human brain natriuretic peptide (human BNP) from the human atrium. Sequence analysis has revealed that it is a 32-amino-acid peptide with the sequence S-P-K-M-V-Q-G-S-G-C-F-G-R-K-M-D-R-I-S-S-S-S-G-L-G-C-K-V-L-R-R-H, which is identical to the C-terminal sequence (77-108) of the human BNP precursor deduced from the cDNA sequence. The sequence of human BNP (77-108) is preceded by Pro75-Arg76 in the human BNP precursor, which is the same processing signal as Pro97-Arg98 of the precursor of atrial natriuretic peptide (ANP). The processing of the BNP precursor occurs in the cardiocyte, although that of the ANP precursor in the cardiocyte is unclear at present.
| Original language | English |
|---|---|
| Pages (from-to) | 341-345 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 259 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 1 1990 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
Cite this
Isolation and sequence determination of human brain natriuretic peptide in human atrium. / Kambayashi, Yoshikazu; Nakao, Kazuwa; Mukoyama, Masashi; Saito, Yoshihiko; Ogawa, Yoshihiro; Shiono, Shozo; Inouye, Ken; Yoshida, Nobuo; Imura, Hiroo.
In: FEBS Letters, Vol. 259, No. 2, 01.01.1990, p. 341-345.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and sequence determination of human brain natriuretic peptide in human atrium
AU - Kambayashi, Yoshikazu
AU - Nakao, Kazuwa
AU - Mukoyama, Masashi
AU - Saito, Yoshihiko
AU - Ogawa, Yoshihiro
AU - Shiono, Shozo
AU - Inouye, Ken
AU - Yoshida, Nobuo
AU - Imura, Hiroo
PY - 1990/1/1
Y1 - 1990/1/1
N2 - We isolated human brain natriuretic peptide (human BNP) from the human atrium. Sequence analysis has revealed that it is a 32-amino-acid peptide with the sequence S-P-K-M-V-Q-G-S-G-C-F-G-R-K-M-D-R-I-S-S-S-S-G-L-G-C-K-V-L-R-R-H, which is identical to the C-terminal sequence (77-108) of the human BNP precursor deduced from the cDNA sequence. The sequence of human BNP (77-108) is preceded by Pro75-Arg76 in the human BNP precursor, which is the same processing signal as Pro97-Arg98 of the precursor of atrial natriuretic peptide (ANP). The processing of the BNP precursor occurs in the cardiocyte, although that of the ANP precursor in the cardiocyte is unclear at present.
AB - We isolated human brain natriuretic peptide (human BNP) from the human atrium. Sequence analysis has revealed that it is a 32-amino-acid peptide with the sequence S-P-K-M-V-Q-G-S-G-C-F-G-R-K-M-D-R-I-S-S-S-S-G-L-G-C-K-V-L-R-R-H, which is identical to the C-terminal sequence (77-108) of the human BNP precursor deduced from the cDNA sequence. The sequence of human BNP (77-108) is preceded by Pro75-Arg76 in the human BNP precursor, which is the same processing signal as Pro97-Arg98 of the precursor of atrial natriuretic peptide (ANP). The processing of the BNP precursor occurs in the cardiocyte, although that of the ANP precursor in the cardiocyte is unclear at present.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025015089&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(90)80043-I
DO - 10.1016/0014-5793(90)80043-I
M3 - Article
C2 - 2136732
AN - SCOPUS:0025015089
VL - 259
SP - 341
EP - 345
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -