Isolation and sequencing of a cDNA clone encoding the 85 kDa human lysosomal sialoglycoprotein (hLGP85) in human metastatic pancreas islet tumor cells

Hideaki Fujita, Yutaka Takata, Akira Kono, Yoshitaka Tanaka, Takeshi Takahashi, Masaru Himeno, Keitaro Kato

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A full length cDNA for a human lysosomal membrane sialoglycoprotein (hLGP85) was isolated as a probe of the cDNA of rat LGP85 (rLGP85) from the cDNA library prepared from total mRNA of QGP-1NL cells, a human pancreatic islet tumor cell with a high metastatic activity. The deduced amino acid sequence shows that hLGP85 consists of 478 amino acid residues (MW. 54,289). The protein has 10 putative N-glycosylation sites and 2 hydrophobic regions at the NH2- and near the COOH-termini, respectively. Thus, both domains probably constitute putative transmembrane domains. It exhibits 86% and 79% sequence similarities in amino acids and nucleic acids to rat lysosomal membrane sialoglycoprotein (rLGP85), respectively. The protein contained the short cytoplasmic tail at the COOH-terminus which does not form the glycine-tyrosine sequence (GY motif), the so-called lysosomal targetting signal.

Original languageEnglish
Pages (from-to)604-611
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume184
Issue number2
DOIs
Publication statusPublished - Apr 30 1992

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Sialoglycoproteins
Islet Cell Adenoma
Rats
Tumors
Pancreas
Complementary DNA
Clone Cells
Cells
Amino Acids
Glycosylation
Membranes
Gene Library
Glycine
Nucleic Acids
Tyrosine
Tail
Amino Acid Sequence
Proteins
Messenger RNA
human SCARB2 protein

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Isolation and sequencing of a cDNA clone encoding the 85 kDa human lysosomal sialoglycoprotein (hLGP85) in human metastatic pancreas islet tumor cells. / Fujita, Hideaki; Takata, Yutaka; Kono, Akira; Tanaka, Yoshitaka; Takahashi, Takeshi; Himeno, Masaru; Kato, Keitaro.

In: Biochemical and Biophysical Research Communications, Vol. 184, No. 2, 30.04.1992, p. 604-611.

Research output: Contribution to journalArticle

Fujita, Hideaki ; Takata, Yutaka ; Kono, Akira ; Tanaka, Yoshitaka ; Takahashi, Takeshi ; Himeno, Masaru ; Kato, Keitaro. / Isolation and sequencing of a cDNA clone encoding the 85 kDa human lysosomal sialoglycoprotein (hLGP85) in human metastatic pancreas islet tumor cells. In: Biochemical and Biophysical Research Communications. 1992 ; Vol. 184, No. 2. pp. 604-611.
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