Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse

Saowanit Tongpim, Kenji Sakai

Research output: Contribution to journalArticle

Abstract

Seven thermotolerant, lactic acid-producing bacteria were isolated from tapioca factory waste and, liquid and solid organic composts in the northeastern Thailand. The)' were able to grow at temperatures ranging from 30 to 60°C, with maximum growth observed at approximately 42 °C, and within a pH ranging of 5.2 to 7.5, with maximum growth observed at approximately pH 6.5. These bacteria were identified as the genus Bacillus based on their phenotypic characteristics. The fermentation of saccharified kitchen refuse by these strains was also evaluated. Using glucoamylase-pretreated model kitchen refuse as a substrate medium, all seven strains produced I.-lactic acid (15.12-24.07 g/1). Among the seven strains tested, strain N15 produced the largest amount of L-lactic acid (24.07 g/1), achieving a 149.3% yield from glucose, 144.9% yield from total sugar, 97% optical activity, 95.5% lactic acid selectivity and 0.31 g/l/h L-lactic acid productivity. By using 16S rDNA sequence analysis, strain N15 displayed 99.45% homology' to Bacillus coagulans.

Original languageEnglish
Pages (from-to)63
Number of pages1
JournalChiang Mai Journal of Science
Volume42
Issue number1
Publication statusPublished - 2015

Fingerprint

Kitchens
lactic acid
Bacillus
Bacilli
Lactic acid
Isolation
Lactic Acid
isolation
Bacteria
bacteria
Optical Activity
Thailand
Solid Waste
Glucan 1,4-alpha-Glucosidase
Optical Rotation
organic solids
Manihot
fermentation
Fermentation
organic liquids

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Mathematics(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse. / Tongpim, Saowanit; Sakai, Kenji.

In: Chiang Mai Journal of Science, Vol. 42, No. 1, 2015, p. 63.

Research output: Contribution to journalArticle

@article{75ace0640e6a4d70ad07dfa6acb5b853,
title = "Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse",
abstract = "Seven thermotolerant, lactic acid-producing bacteria were isolated from tapioca factory waste and, liquid and solid organic composts in the northeastern Thailand. The)' were able to grow at temperatures ranging from 30 to 60°C, with maximum growth observed at approximately 42 °C, and within a pH ranging of 5.2 to 7.5, with maximum growth observed at approximately pH 6.5. These bacteria were identified as the genus Bacillus based on their phenotypic characteristics. The fermentation of saccharified kitchen refuse by these strains was also evaluated. Using glucoamylase-pretreated model kitchen refuse as a substrate medium, all seven strains produced I.-lactic acid (15.12-24.07 g/1). Among the seven strains tested, strain N15 produced the largest amount of L-lactic acid (24.07 g/1), achieving a 149.3{\%} yield from glucose, 144.9{\%} yield from total sugar, 97{\%} optical activity, 95.5{\%} lactic acid selectivity and 0.31 g/l/h L-lactic acid productivity. By using 16S rDNA sequence analysis, strain N15 displayed 99.45{\%} homology' to Bacillus coagulans.",
author = "Saowanit Tongpim and Kenji Sakai",
year = "2015",
language = "English",
volume = "42",
pages = "63",
journal = "Chiang Mai Journal of Science",
issn = "0125-2526",
publisher = "Chiang Mai University",
number = "1",

}

TY - JOUR

T1 - Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse

AU - Tongpim, Saowanit

AU - Sakai, Kenji

PY - 2015

Y1 - 2015

N2 - Seven thermotolerant, lactic acid-producing bacteria were isolated from tapioca factory waste and, liquid and solid organic composts in the northeastern Thailand. The)' were able to grow at temperatures ranging from 30 to 60°C, with maximum growth observed at approximately 42 °C, and within a pH ranging of 5.2 to 7.5, with maximum growth observed at approximately pH 6.5. These bacteria were identified as the genus Bacillus based on their phenotypic characteristics. The fermentation of saccharified kitchen refuse by these strains was also evaluated. Using glucoamylase-pretreated model kitchen refuse as a substrate medium, all seven strains produced I.-lactic acid (15.12-24.07 g/1). Among the seven strains tested, strain N15 produced the largest amount of L-lactic acid (24.07 g/1), achieving a 149.3% yield from glucose, 144.9% yield from total sugar, 97% optical activity, 95.5% lactic acid selectivity and 0.31 g/l/h L-lactic acid productivity. By using 16S rDNA sequence analysis, strain N15 displayed 99.45% homology' to Bacillus coagulans.

AB - Seven thermotolerant, lactic acid-producing bacteria were isolated from tapioca factory waste and, liquid and solid organic composts in the northeastern Thailand. The)' were able to grow at temperatures ranging from 30 to 60°C, with maximum growth observed at approximately 42 °C, and within a pH ranging of 5.2 to 7.5, with maximum growth observed at approximately pH 6.5. These bacteria were identified as the genus Bacillus based on their phenotypic characteristics. The fermentation of saccharified kitchen refuse by these strains was also evaluated. Using glucoamylase-pretreated model kitchen refuse as a substrate medium, all seven strains produced I.-lactic acid (15.12-24.07 g/1). Among the seven strains tested, strain N15 produced the largest amount of L-lactic acid (24.07 g/1), achieving a 149.3% yield from glucose, 144.9% yield from total sugar, 97% optical activity, 95.5% lactic acid selectivity and 0.31 g/l/h L-lactic acid productivity. By using 16S rDNA sequence analysis, strain N15 displayed 99.45% homology' to Bacillus coagulans.

UR - http://www.scopus.com/inward/record.url?scp=84922041598&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922041598&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84922041598

VL - 42

SP - 63

JO - Chiang Mai Journal of Science

JF - Chiang Mai Journal of Science

SN - 0125-2526

IS - 1

ER -