Isolation of a carp complement protein homologous to mammalian factor D

Yano Tomoki, Miki Nakao

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Factor D of carp (Cyprinus carpio) complement was purified to apparent homogeneity by a 4-step Chromatographie procedure and examined for physicochemical and functional properties. Carp factor D proved to be an α-globulin with a molecular mass of 29 kDa and the serum concentration was estimated to be 6 μg/ml. The NH2-terminal amino acid sequence (30 residues including five unidentified positions) of carp factor D showed high homologies (57-60%) to those of mammalian factor D. Neither functional compatibility nor common antigenicity was observed between carp and human factor D. This report is apparently the first description on the factor D molecule in a non-mammalian species.

Original languageEnglish
Pages (from-to)337-342
Number of pages6
JournalMolecular Immunology
Volume31
Issue number5
DOIs
Publication statusPublished - Jan 1 1994

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

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