Isolation of a human X chromosome-linked gene essential for progression from G1 to S phase of the cell cycle

Takeshi Sekiguchi, Michihiro C. Yoshida, Mutsuo Sekiguchi, Takeharu Nishimoto

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The tsBN462 cell line, a temperature-sensitive (ts) mutant isolated from the hamster cell line, BHK21/13, cannot progress into S phase at 39.5 °C, following the release from isoleucine deprivation. The mutant cells were transfected with high molecular weight (HMW) DNA from human KB cells, and several human DNA bands were found to be conserved through three cycles of ts+ transformation. Conserved human DNA was isolated from the cosmid library of the secondary ts+ transformant (K-1-1), using 32P-labelled total human DNA as a probe. The isolated human DNA covers about 70 kb of human DNA flanked with hamster DNA, and originates from the human X chromosome. The middle part (56 kb) of the isolated human DNA was conserved through the primary, secondary and tertiary ts+ transformation, without gross rearrangement.

Original languageEnglish
Pages (from-to)395-407
Number of pages13
JournalExperimental Cell Research
Volume169
Issue number2
DOIs
Publication statusPublished - Apr 1987

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Chromosomes, Human, X
X-Linked Genes
S Phase
Cell Cycle
DNA
Temperature
Cricetinae
KB Cells
Cell Line
Cosmids
Isoleucine
Molecular Weight

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Isolation of a human X chromosome-linked gene essential for progression from G1 to S phase of the cell cycle. / Sekiguchi, Takeshi; Yoshida, Michihiro C.; Sekiguchi, Mutsuo; Nishimoto, Takeharu.

In: Experimental Cell Research, Vol. 169, No. 2, 04.1987, p. 395-407.

Research output: Contribution to journalArticle

Sekiguchi, Takeshi ; Yoshida, Michihiro C. ; Sekiguchi, Mutsuo ; Nishimoto, Takeharu. / Isolation of a human X chromosome-linked gene essential for progression from G1 to S phase of the cell cycle. In: Experimental Cell Research. 1987 ; Vol. 169, No. 2. pp. 395-407.
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