Isolation of a new peroxisome-deficient CHO cell mutant defective in peroxisome targeting signal-1 receptor

Toshiro Tsukamoto, Akemi Bogaki, Kanji Okumoto, Keita Tateishi, Yukio Fujiki, Nobuyuki Shimozawa, Yasuyuki Suzuki, Naomi Kondo, Takashi Osumi

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35 Citations (Scopus)

Abstract

For the study of mechanism of peroxisome biogenesis, we attempted to isolate CHO cell mutants deficient in peroxisome biogenesis. We used as the parent strain a stable CHO transformant of rat PEX2 (formerly named peroxisome assembly factor-1) cDNA, to avoid unusually frequent isolation of Pex2 mutants. Among the three peroxisome-deficient mutants obtained, ZP102 was a new CHO mutant of complementation group 2, and was restored for peroxisome assembly by the transfection of human PEX5 (formerly called PXR1 or PTS1R) cDNA. This approach would facilitate the isolation of new complementation gorups of peroxisome-deficient CHO mutants and the identification of essential genes for peroxisome biogenesis.

Original languageEnglish
Pages (from-to)402-406
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume230
Issue number2
DOIs
Publication statusPublished - Jan 13 1997

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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