Abstract
Four cDNA fragments, whose corresponding mRNAs were accumulated in growth-arrested confluent but not in growing sub-confluent rat 3Y1 cells, were isolated by the mRNA differential display method. Sequencing of the four cDNA fragments indicated that one of them was highly homologous to 3' untranslated DNA region of mouse growth-arrest specific cDNA, while the other three (named confluent 3Y1 cell-associated No. 1 (ccal), cca2 and cca3) were novel. Of the three novel cDNAs, we presented some characteristics of cca2 cDNA: the cDNA consisted of 1795 nucleotides with a deduced open reading frame (ORF) encoding a protein of 338 amino acids, which showed a 35% identity with rat type IV 3β-hydroxysteroid dehydrogenase. The CCA2 protein, with a molecular weight of 38 kDa, was accumulated in 3Y1 cells under growth arrest conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 145-150 |
| Number of pages | 6 |
| Journal | Biochimica et Biophysica Acta - Gene Structure and Expression |
| Volume | 1352 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - May 30 1997 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Genetics
- Structural Biology
- Biophysics
Cite this
Isolation of a novel cDNA whose corresponding mRNA is accumulated in growth-arrested confluent but not in growing sub-confluent rat 3Y1 cells. / Hayashi, Yasuyuki; Kiyono, Tohru; Fujita, Masatoshi; Ishibashi, Masahide.
In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1352, No. 2, 30.05.1997, p. 145-150.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Isolation of a novel cDNA whose corresponding mRNA is accumulated in growth-arrested confluent but not in growing sub-confluent rat 3Y1 cells
AU - Hayashi, Yasuyuki
AU - Kiyono, Tohru
AU - Fujita, Masatoshi
AU - Ishibashi, Masahide
PY - 1997/5/30
Y1 - 1997/5/30
N2 - Four cDNA fragments, whose corresponding mRNAs were accumulated in growth-arrested confluent but not in growing sub-confluent rat 3Y1 cells, were isolated by the mRNA differential display method. Sequencing of the four cDNA fragments indicated that one of them was highly homologous to 3' untranslated DNA region of mouse growth-arrest specific cDNA, while the other three (named confluent 3Y1 cell-associated No. 1 (ccal), cca2 and cca3) were novel. Of the three novel cDNAs, we presented some characteristics of cca2 cDNA: the cDNA consisted of 1795 nucleotides with a deduced open reading frame (ORF) encoding a protein of 338 amino acids, which showed a 35% identity with rat type IV 3β-hydroxysteroid dehydrogenase. The CCA2 protein, with a molecular weight of 38 kDa, was accumulated in 3Y1 cells under growth arrest conditions.
AB - Four cDNA fragments, whose corresponding mRNAs were accumulated in growth-arrested confluent but not in growing sub-confluent rat 3Y1 cells, were isolated by the mRNA differential display method. Sequencing of the four cDNA fragments indicated that one of them was highly homologous to 3' untranslated DNA region of mouse growth-arrest specific cDNA, while the other three (named confluent 3Y1 cell-associated No. 1 (ccal), cca2 and cca3) were novel. Of the three novel cDNAs, we presented some characteristics of cca2 cDNA: the cDNA consisted of 1795 nucleotides with a deduced open reading frame (ORF) encoding a protein of 338 amino acids, which showed a 35% identity with rat type IV 3β-hydroxysteroid dehydrogenase. The CCA2 protein, with a molecular weight of 38 kDa, was accumulated in 3Y1 cells under growth arrest conditions.
UR - http://www.scopus.com/inward/record.url?scp=0030900803&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030900803&partnerID=8YFLogxK
U2 - 10.1016/S0167-4781(97)00051-1
DO - 10.1016/S0167-4781(97)00051-1
M3 - Article
C2 - 9199244
AN - SCOPUS:0030900803
VL - 1352
SP - 145
EP - 150
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 2
ER -