Isolation of a novel cDNA whose corresponding mRNA is accumulated in growth-arrested confluent but not in growing sub-confluent rat 3Y1 cells

Yasuyuki Hayashi, Tohru Kiyono, Masatoshi Fujita, Masahide Ishibashi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Four cDNA fragments, whose corresponding mRNAs were accumulated in growth-arrested confluent but not in growing sub-confluent rat 3Y1 cells, were isolated by the mRNA differential display method. Sequencing of the four cDNA fragments indicated that one of them was highly homologous to 3' untranslated DNA region of mouse growth-arrest specific cDNA, while the other three (named confluent 3Y1 cell-associated No. 1 (ccal), cca2 and cca3) were novel. Of the three novel cDNAs, we presented some characteristics of cca2 cDNA: the cDNA consisted of 1795 nucleotides with a deduced open reading frame (ORF) encoding a protein of 338 amino acids, which showed a 35% identity with rat type IV 3β-hydroxysteroid dehydrogenase. The CCA2 protein, with a molecular weight of 38 kDa, was accumulated in 3Y1 cells under growth arrest conditions.

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1352
Issue number2
DOIs
Publication statusPublished - May 30 1997
Externally publishedYes

Fingerprint

Rats
Complementary DNA
Messenger RNA
Growth
3-Hydroxysteroid Dehydrogenases
3' Untranslated Regions
Gene Expression Profiling
Open Reading Frames
Proteins
Nucleotides
Molecular Weight
Molecular weight
Display devices
Amino Acids
DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Isolation of a novel cDNA whose corresponding mRNA is accumulated in growth-arrested confluent but not in growing sub-confluent rat 3Y1 cells. / Hayashi, Yasuyuki; Kiyono, Tohru; Fujita, Masatoshi; Ishibashi, Masahide.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1352, No. 2, 30.05.1997, p. 145-150.

Research output: Contribution to journalArticle

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