Isolation of a thermophilic poly-L-lactide degrading bacterium from compost and its enzymatic characterization

Kenji Sakai; Hiroyuki Kawano; Akihiko Iwami; Masakazu Nakamura; Mitsuaki Moriguchi

doi:10.1016/S1389-1723(01)80266-8

Isolation of a thermophilic poly-L-lactide degrading bacterium from compost and its enzymatic characterization

Kenji Sakai, Hiroyuki Kawano, Akihiko Iwami, Masakazu Nakamura, Mitsuaki Moriguchi

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

Thermophilic poly-L-lactide-degrading bacteria were isolated from a garbage fermentor. One of the isolates, strain PL21, was identified as Bacillus smithii based on its physiological properties, sugar assimilation pattern, and partial 16S rDNA sequence. The degradation activity of poly-L-lactide exibited by the culture fluid was parallel to the esterase activity, and the purified enzyme was active against various fatty acid esters and poly-L-lactide, at 60°C and pH 5.

Original language	English
Pages (from-to)	298-300
Number of pages	3
Journal	Journal of Bioscience and Bioengineering
Volume	92
Issue number	3
DOIs	https://doi.org/10.1016/S1389-1723(01)80266-8
Publication status	Published - Jan 1 2001
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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AU - Sakai, Kenji

AU - Kawano, Hiroyuki

AU - Iwami, Akihiko

AU - Nakamura, Masakazu

AU - Moriguchi, Mitsuaki

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AB - Thermophilic poly-L-lactide-degrading bacteria were isolated from a garbage fermentor. One of the isolates, strain PL21, was identified as Bacillus smithii based on its physiological properties, sugar assimilation pattern, and partial 16S rDNA sequence. The degradation activity of poly-L-lactide exibited by the culture fluid was parallel to the esterase activity, and the purified enzyme was active against various fatty acid esters and poly-L-lactide, at 60°C and pH 5.

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