Isolation of cDNA encoding the constant region of the immuoglobulin heavy-chain from common carp (Cyprinus carpio L.)

Miki Nakao, Tadaaki Moritomo, Mitsuru Tomana, Kazuhiro Fujiki, Tomoki Yano

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Complementary DNA fragments encoding the constant region (CH) of immunoglobulin heavy chain were isolated from spleen of carp (Cyprinus carpio L.) by reverse transcription-polymerase chain reaction (RT-PCR) using degenerated primers corresponding to the conserved amino acid sequences in the J-segment of the variable region, and in CH4. Sequences encoding CH4 and transmembrane domains were also obtained by a rapid amplification of cDNA end method (3′-RACE). Primary structure of the entire carp CH domain of the secreted form contained well conserved cysteine and tryptophan residues and showed 30% to 42% identity with that of other teleost species. Sequence analysis of membrane-bound CH domain revealed that the carp transmembrane domain is connected to the end of CH3, splicing out the entire CH4 exon, as in other teleosts. Interestingly, three distinct partial CH sequences which share 72-95% identity at amino acid level were obtained from a single carp. This strongly indicates the presence of duplicated CH genes, which are more divergent than those in Atlantic salmon, which carries two isotypic Ig H genes. Southern hybridisation using carp erythrocyte DNA also revealed two distinct CH genes indicating that each of the duplicated CH genes is disomicly inherited. It seems likely that the duplication in carp occurred as whole Ig H genes, in association with ancient tetraploidisation, rather than by tandem duplication, as reported for another tetraploidised teleost, Atlantic salmon.

Original languageEnglish
Pages (from-to)425-434
Number of pages10
JournalFish and Shellfish Immunology
Volume8
Issue number6
DOIs
Publication statusPublished - Jan 1 1998

Fingerprint

Cyprinus carpio
carp
Complementary DNA
Genes
teleost
gene
genes
amino acid
Salmo salar
Amino Acids
DNA
Immunoglobulin Heavy Chains
immunoglobulin heavy chains
Polymerase chain reaction
Transcription
complementary DNA
rapid amplification of cDNA ends
Tryptophan
polymerase chain reaction
Cysteine

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Cite this

Isolation of cDNA encoding the constant region of the immuoglobulin heavy-chain from common carp (Cyprinus carpio L.). / Nakao, Miki; Moritomo, Tadaaki; Tomana, Mitsuru; Fujiki, Kazuhiro; Yano, Tomoki.

In: Fish and Shellfish Immunology, Vol. 8, No. 6, 01.01.1998, p. 425-434.

Research output: Contribution to journalArticle

Nakao, Miki ; Moritomo, Tadaaki ; Tomana, Mitsuru ; Fujiki, Kazuhiro ; Yano, Tomoki. / Isolation of cDNA encoding the constant region of the immuoglobulin heavy-chain from common carp (Cyprinus carpio L.). In: Fish and Shellfish Immunology. 1998 ; Vol. 8, No. 6. pp. 425-434.
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