Isolation of compound microsatellite markers for the endangered plant Neolitsea sericea (Lauraceae)

Sheng Nan Zhai, Xiao Ling Yan, Koh Nakamura, Misako Mishima, Ying Xiong Qiu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Premise of the study: The development of compound microsatellite markers was conducted in Neolitsea sericea to investigate genetic diversity and population genetic structure of this endangered insular species Methods and Results: Using the compound microsatellite marker technique, 10 compound microsatellite markers that were successfully amplified showed polymorphism when assessed in 55 individuals from two populations in East China and Japan. Overall, the number of alleles ranged from 3 to 17, with an average of 7.9 alleles per locus. In addition, these primers could be easily amplified in Neolitsea aurata var. paraciculata and N. aurata var. chekiangensi s. Conclusions: The highly polymorphic markers developed and characterized in this study will be useful for population genetic studies of N. sericea

Original languageEnglish
JournalAmerican Journal of Botany
Volume97
Issue number12
DOIs
Publication statusPublished - Dec 1 2010

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Neolitsea sericea
Lauraceae
endangered species
Microsatellite Repeats
Population Genetics
microsatellite repeats
population genetics
Neolitsea
Alleles
alleles
Endangered Species
allele
Genetic Structures
China
Japan
genetic polymorphism
genetic structure
genetic variation
loci
polymorphism

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Plant Science

Cite this

Isolation of compound microsatellite markers for the endangered plant Neolitsea sericea (Lauraceae). / Zhai, Sheng Nan; Yan, Xiao Ling; Nakamura, Koh; Mishima, Misako; Qiu, Ying Xiong.

In: American Journal of Botany, Vol. 97, No. 12, 01.12.2010.

Research output: Contribution to journalArticle

Zhai, Sheng Nan ; Yan, Xiao Ling ; Nakamura, Koh ; Mishima, Misako ; Qiu, Ying Xiong. / Isolation of compound microsatellite markers for the endangered plant Neolitsea sericea (Lauraceae). In: American Journal of Botany. 2010 ; Vol. 97, No. 12.
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