Isolation of embryonic hematopoietic niche cells by flow cytometry and laser capture microdissection

Daisuke Sugiyama, Tatsuya Sasaki

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Hematopoietic stem cells (HSCs) can differentiate into several types of hematopoietic cells, such as erythrocytes, megakaryocytes, lymphocytes, neutrophils, or macrophages, and also undergo self-renewal to sustain hematopoiesis throughout an organism's lifetime. HSCs emerge and expand during mouse embryogenesis. HSC regulation is governed by two types of activity: intrinsic activity programmed primarily by cell autonomous gene expression, and extrinsic factors, which originate from the so-called niche cells surrounding HSCs. Previously, we reported that endothelial niche cells regulate HSC generationat aorta-gonad- mesonephros region and placenta, and that hepatoblastic niche cells regulate HSC differentiation in mouse embryonic liver. In the course of those studies, we employed immunohistochemistry,flow cytometry, and the laser capture microdissection system to assess embryonic regulation of the mouse hematopoietic niche.

Original languageEnglish
Title of host publicationStem Cell Niche
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages57-65
Number of pages9
ISBN (Print)9781627035071
DOIs
Publication statusPublished - Jan 1 2013

Publication series

NameMethods in Molecular Biology
Volume1035
ISSN (Print)1064-3745

Fingerprint

Laser Capture Microdissection
Hematopoietic Stem Cells
Flow Cytometry
Mesonephros
Megakaryocytes
Hematopoiesis
Gonads
Placenta
Embryonic Development
Aorta
Cell Differentiation
Neutrophils
Endothelial Cells
Erythrocytes
Immunohistochemistry
Macrophages
Lymphocytes
Gene Expression
Liver

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Sugiyama, D., & Sasaki, T. (2013). Isolation of embryonic hematopoietic niche cells by flow cytometry and laser capture microdissection. In Stem Cell Niche: Methods and Protocols (pp. 57-65). (Methods in Molecular Biology; Vol. 1035). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-508-8_6

Isolation of embryonic hematopoietic niche cells by flow cytometry and laser capture microdissection. / Sugiyama, Daisuke; Sasaki, Tatsuya.

Stem Cell Niche: Methods and Protocols. Humana Press Inc., 2013. p. 57-65 (Methods in Molecular Biology; Vol. 1035).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sugiyama, D & Sasaki, T 2013, Isolation of embryonic hematopoietic niche cells by flow cytometry and laser capture microdissection. in Stem Cell Niche: Methods and Protocols. Methods in Molecular Biology, vol. 1035, Humana Press Inc., pp. 57-65. https://doi.org/10.1007/978-1-62703-508-8_6
Sugiyama D, Sasaki T. Isolation of embryonic hematopoietic niche cells by flow cytometry and laser capture microdissection. In Stem Cell Niche: Methods and Protocols. Humana Press Inc. 2013. p. 57-65. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-508-8_6
Sugiyama, Daisuke ; Sasaki, Tatsuya. / Isolation of embryonic hematopoietic niche cells by flow cytometry and laser capture microdissection. Stem Cell Niche: Methods and Protocols. Humana Press Inc., 2013. pp. 57-65 (Methods in Molecular Biology).
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