Abstract
Hematopoietic stem cells (HSCs) can differentiate into several types of hematopoietic cells, such as erythrocytes, megakaryocytes, lymphocytes, neutrophils, or macrophages, and also undergo self-renewal to sustain hematopoiesis throughout an organism's lifetime. HSCs emerge and expand during mouse embryogenesis. HSC regulation is governed by two types of activity: intrinsic activity programmed primarily by cell autonomous gene expression, and extrinsic factors, which originate from the so-called niche cells surrounding HSCs. Previously, we reported that endothelial niche cells regulate HSC generationat aorta-gonad- mesonephros region and placenta, and that hepatoblastic niche cells regulate HSC differentiation in mouse embryonic liver. In the course of those studies, we employed immunohistochemistry,flow cytometry, and the laser capture microdissection system to assess embryonic regulation of the mouse hematopoietic niche.
| Original language | English |
|---|---|
| Title of host publication | Stem Cell Niche |
| Subtitle of host publication | Methods and Protocols |
| Publisher | Humana Press Inc. |
| Pages | 57-65 |
| Number of pages | 9 |
| ISBN (Print) | 9781627035071 |
| DOIs | |
| Publication status | Published - Jan 1 2013 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1035 |
| ISSN (Print) | 1064-3745 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
Cite this
Isolation of embryonic hematopoietic niche cells by flow cytometry and laser capture microdissection. / Sugiyama, Daisuke; Sasaki, Tatsuya.
Stem Cell Niche: Methods and Protocols. Humana Press Inc., 2013. p. 57-65 (Methods in Molecular Biology; Vol. 1035).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Isolation of embryonic hematopoietic niche cells by flow cytometry and laser capture microdissection
AU - Sugiyama, Daisuke
AU - Sasaki, Tatsuya
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Hematopoietic stem cells (HSCs) can differentiate into several types of hematopoietic cells, such as erythrocytes, megakaryocytes, lymphocytes, neutrophils, or macrophages, and also undergo self-renewal to sustain hematopoiesis throughout an organism's lifetime. HSCs emerge and expand during mouse embryogenesis. HSC regulation is governed by two types of activity: intrinsic activity programmed primarily by cell autonomous gene expression, and extrinsic factors, which originate from the so-called niche cells surrounding HSCs. Previously, we reported that endothelial niche cells regulate HSC generationat aorta-gonad- mesonephros region and placenta, and that hepatoblastic niche cells regulate HSC differentiation in mouse embryonic liver. In the course of those studies, we employed immunohistochemistry,flow cytometry, and the laser capture microdissection system to assess embryonic regulation of the mouse hematopoietic niche.
AB - Hematopoietic stem cells (HSCs) can differentiate into several types of hematopoietic cells, such as erythrocytes, megakaryocytes, lymphocytes, neutrophils, or macrophages, and also undergo self-renewal to sustain hematopoiesis throughout an organism's lifetime. HSCs emerge and expand during mouse embryogenesis. HSC regulation is governed by two types of activity: intrinsic activity programmed primarily by cell autonomous gene expression, and extrinsic factors, which originate from the so-called niche cells surrounding HSCs. Previously, we reported that endothelial niche cells regulate HSC generationat aorta-gonad- mesonephros region and placenta, and that hepatoblastic niche cells regulate HSC differentiation in mouse embryonic liver. In the course of those studies, we employed immunohistochemistry,flow cytometry, and the laser capture microdissection system to assess embryonic regulation of the mouse hematopoietic niche.
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UR - http://www.scopus.com/inward/citedby.url?scp=84934439043&partnerID=8YFLogxK
U2 - 10.1007/978-1-62703-508-8_6
DO - 10.1007/978-1-62703-508-8_6
M3 - Chapter
SN - 9781627035071
T3 - Methods in Molecular Biology
SP - 57
EP - 65
BT - Stem Cell Niche
PB - Humana Press Inc.
ER -