Isolation of enterocin SE-K4-encoding plasmid and a high enterocin SE-K4 producing strain of enterococcus faecalis K-4

Katsumi Doi, Tomoko Eguchi, Seong Hyun Choi, Atsushi Iwatake, Sadahiro Ohmomo, Seiya Ogata

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Abstract

Enterococcus faecalis K-4, which produces a class IIa bacteriocin, enterocin SE-K4, carries two plasmids, pEK4S (approximately 60 kb) and pEK4L (approximately 75 kb). Plasmid-curing experiments showed that pEK4S was involved in the production of and immunity to enterocin SE-K4 in strain K-4. A derivative strain, M6, with pEK4S produced a higher amount of enterocin SE-K4 than the parental strain K-4, although its growth rate was lower than that of parental strain K-4. Phenotypic changes in strain M6 are attributed to an increase in plasmid copy number.

Original languageEnglish
Pages (from-to)434-436
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume93
Issue number4
DOIs
Publication statusPublished - Jan 1 2002

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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