Isolation of ginsenoside Rb1 from Kalopanax pictus by eastern blotting using anti-ginsenoside Rb1 monoclonal antibody

Hiroyuki Tanaka, Noriko Fukuda, Shoji Yahara, Susumu Isoda, Chun Su Yuan, Yukihiro Shoyama

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Araliaceous species containing ginsenosides, the major active component in Panax species, were surveyed using ELISA and eastern blotting with anti-ginsenoside Rb1 monoclonal antibody. Immunoassay-guided fractionation of the methanol-soluble extracts of Araliaceous species led to the isolation of a known compound in the bark of Kalopanax pictus Nakai. The known compound was identified as ginsenoside Rb1 by spectroscopic methods and by comparison with an authentic sample. The result provided evidence that a combination of two immunoassays using monoclonal antibodies against ginsenosides is a powerful means of surveying new ginsenoside resources.

Original languageEnglish
Pages (from-to)255-258
Number of pages4
JournalPhytotherapy Research
Volume19
Issue number3
DOIs
Publication statusPublished - Mar 1 2005

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Kalopanax
Ginsenosides
Monoclonal Antibodies
Immunoassay
Panax
Surveying
Fractionation
Methanol
Enzyme-Linked Immunosorbent Assay
ginsenoside Rb1

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Isolation of ginsenoside Rb1 from Kalopanax pictus by eastern blotting using anti-ginsenoside Rb1 monoclonal antibody. / Tanaka, Hiroyuki; Fukuda, Noriko; Yahara, Shoji; Isoda, Susumu; Yuan, Chun Su; Shoyama, Yukihiro.

In: Phytotherapy Research, Vol. 19, No. 3, 01.03.2005, p. 255-258.

Research output: Contribution to journalArticle

Tanaka, Hiroyuki ; Fukuda, Noriko ; Yahara, Shoji ; Isoda, Susumu ; Yuan, Chun Su ; Shoyama, Yukihiro. / Isolation of ginsenoside Rb1 from Kalopanax pictus by eastern blotting using anti-ginsenoside Rb1 monoclonal antibody. In: Phytotherapy Research. 2005 ; Vol. 19, No. 3. pp. 255-258.
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