Isolation of microsatellite markers from Pinus densiflora Sieb. et Zucc. using a dual PCR technique

A. Watanabe, M. G. Iwaizumi, M. Ubukata, T. Kondo, C. Lian, T. Hogetsu

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

We developed seven microsatellite loci from Pinus densiflora using a dual polymerase chain reaction (PCR) technique. Of 186 clones from a library based on suppression PCR, 127 contained microsatellite sequences. Of these, 43 candidates were determined sequences of both flanking regions, and 16 regions from this group were chosen as development markers. Seven of these primer pairs successfully amplified polymorphic single loci among 83 resistant trees against pine wood nematode. The observed heterozygosity of the seven microsatellite markers ranged from 0.247 to 0.843. Mendelian inheritance was confirmed using megagametophytes.

Original languageEnglish
Pages (from-to)80-82
Number of pages3
JournalMolecular Ecology Notes
Volume6
Issue number1
DOIs
Publication statusPublished - Mar 1 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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