Isolation of rat mitochondrial transcription factor A (r-Tfam) cDNA

Hidetoshi Inagaki, Toshihiko Hayashi, Yuichi Matsushima, Kong Hua Lin, Sumio Maeda, Shigeyuki Ichihara, Yasuo Kitagawa, Takao Saito

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

We have isolated rat mitochondrial transcription factor A (Tfam; formerly known as mtTFA) cDNA clones from a rat cerebellum cDNA library using human Tfam cDNA as a probe. The deduced amino acid sequence of r-Tfam shows 62% and 89% overall identity to human and mouse Tfam, respectively. We also show the presence of two r-Tfam isoforms in testis as for mouse. Our findings suggest that the mechanisms underlying transcription of mitochondrial genes are conserved among rat, mouse, and human.

Original languageEnglish
Pages (from-to)131-135
Number of pages5
JournalMitochondrial DNA
Volume11
Issue number1-2
DOIs
Publication statusPublished - 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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