Isolation of rat mitochondrial transcription factor A (r-Tfam) cDNA

Hidetoshi Inagaki, Toshihiko Hayashi, Yuichi Matsushima, Kong Hua Lin, Sumio Maeda, Shigeyuki Ichihara, Yasuo Kitagawa, Takao Saito

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We have isolated rat mitochondrial transcription factor A (Tfam; formerly known as mtTFA) cDNA clones from a rat cerebellum cDNA library using human Tfam cDNA as a probe. The deduced amino acid sequence of r-Tfam shows 62% and 89% overall identity to human and mouse Tfam, respectively. We also show the presence of two r-Tfam isoforms in testis as for mouse. Our findings suggest that the mechanisms underlying transcription of mitochondrial genes are conserved among rat, mouse, and human.

Original languageEnglish
Pages (from-to)131-135
Number of pages5
JournalMitochondrial DNA
Volume11
Issue number1-2
DOIs
Publication statusPublished - Dec 1 2000

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Complementary DNA
Mitochondrial Genes
Gene Library
Cerebellum
Testis
Amino Acid Sequence
Protein Isoforms
Clone Cells
rat Tfam protein

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Inagaki, H., Hayashi, T., Matsushima, Y., Lin, K. H., Maeda, S., Ichihara, S., ... Saito, T. (2000). Isolation of rat mitochondrial transcription factor A (r-Tfam) cDNA. Mitochondrial DNA, 11(1-2), 131-135. https://doi.org/10.3109/10425170009033980

Isolation of rat mitochondrial transcription factor A (r-Tfam) cDNA. / Inagaki, Hidetoshi; Hayashi, Toshihiko; Matsushima, Yuichi; Lin, Kong Hua; Maeda, Sumio; Ichihara, Shigeyuki; Kitagawa, Yasuo; Saito, Takao.

In: Mitochondrial DNA, Vol. 11, No. 1-2, 01.12.2000, p. 131-135.

Research output: Contribution to journalArticle

Inagaki, H, Hayashi, T, Matsushima, Y, Lin, KH, Maeda, S, Ichihara, S, Kitagawa, Y & Saito, T 2000, 'Isolation of rat mitochondrial transcription factor A (r-Tfam) cDNA', Mitochondrial DNA, vol. 11, no. 1-2, pp. 131-135. https://doi.org/10.3109/10425170009033980
Inagaki, Hidetoshi ; Hayashi, Toshihiko ; Matsushima, Yuichi ; Lin, Kong Hua ; Maeda, Sumio ; Ichihara, Shigeyuki ; Kitagawa, Yasuo ; Saito, Takao. / Isolation of rat mitochondrial transcription factor A (r-Tfam) cDNA. In: Mitochondrial DNA. 2000 ; Vol. 11, No. 1-2. pp. 131-135.
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