Abstract
Two fragments that could function as replicational cue elements were isolated from a genomic DNA digest of Aspergillus oryzae on the basis of abnormal behavior in polyacrylamide gel electrophoresis. The vector used in this study contained a scaffold-associated region of the Drosophila melanogaster ftz gene to provide nuclear retention. Neither fragment contained a yeast ARS consensus sequence or an eukaryotic topoisomerase II binding sequence. One of the fragments showed sequence homology with the mitochondrial replication origin of Candida utilis and a portion of mitochondrial DNA of Aspergillus nidulans. This plasmid carrying the cue fragment could also replicate in HeLa and NIH3T3 cells.
Original language | English |
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Pages (from-to) | 13-19 |
Number of pages | 7 |
Journal | Molecular biology reports |
Volume | 27 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 1 2000 |
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics