Isolation of suppressor mutants of phosphatidylinositol 3-phosphate 5-kinase deficient cells in schizosaccharomyce pombe

Masayuki Onishi, Yoko Nakamura, Takako Koga, Kaoru Takegawa, Yasuhisa Fukui

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The ste12+ gene of Schizosaccharomyces pombe codes for a phosphatidylinositol (PI) 3-phosphate 5′-kinase, which is required for efficient mating. Suppressor mutants for sterility of ste12Δ cells were screened for. Most of the mutant genes turned out to be recessive. Six genes were cloned and the open reading frames responsible for the suppressor activity were identified. They included genes coding for proteins with domains homologous to calcium transporters, casein kinase II, UBC13, AMSH, Vps23p, and Vps27p of Saccharomyces cerevisiae. Disruption of these genes resulted in suppression of the defects of the ste12Δ cells, including low mating efficiency and formation of large vacuoles. Since many of these gene products are homologous to the proteins involved in vesicle transport, sterility caused by inactivation of ste12 may be due to a disordered vesicle transport system.

Original languageEnglish
Pages (from-to)1772-1779
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number8
DOIs
Publication statusPublished - Jan 1 2003

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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