Isolation of thermophilic l-lactic acid producing bacteria showing homo-fermentative manner under high aeration condition

Saowanit Tongpim, Ratchanu Meidong, Pramod Poudel, Satoshi Yoshino, Yuki Okugawa, Yukihiro Tashiro, Masayuki Taniguchi, Kenji Sakai

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

By applying non-sterile open fermentation of food waste, various thermotolerant l-lactic acid-producing bacteria were isolated and identified. The predominant bacterial isolates showing higher accumulation of l-lactic acid belong to 3 groups of Bacillus coagulans, according to their 16S rRNA gene sequence similarities. B.coagulans strains M21 and M36 produced high amounts of l-lactic acid of high optical purity and lactic acid selectivity in model kitchen refuse medium and glucose-yeast extract-peptone medium. Other thermotolerant isolates resembling to Bacillus humi, B. ruris, B.subtilis, B. niacini and B. soli were also identified. These bacteria produced low amounts of l-lactic acid of more than 99% optical purity. All isolated strains showed the highest growth rate at temperatures around 55-60°C. They showed unique responses to various oxygen supply conditions. The majority of isolates produced l-lactic acid at a low overall oxygen transfer coefficient (KLa); however, acetic acid was produced instead of l-lactic acid at a high KLa. B.coagulans M21 was the only strain that produced high, consistent, and reproducible amounts of optically pure l-lactic acid (>99% optical purity) under high and low KLa conditions in a homo-fermentative manner.

Original languageEnglish
Pages (from-to)318-324
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume117
Issue number3
DOIs
Publication statusPublished - Mar 1 2014

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Lactic acid
Lactic Acid
Bacteria
Bacilli
Oxygen
Oxygen supply
Peptones
Kitchens
rRNA Genes
Acetic acid
Acetic Acid
Yeast
Fermentation
Bacillus
Glucose
Genes
Yeasts
Food
Temperature
Growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Isolation of thermophilic l-lactic acid producing bacteria showing homo-fermentative manner under high aeration condition. / Tongpim, Saowanit; Meidong, Ratchanu; Poudel, Pramod; Yoshino, Satoshi; Okugawa, Yuki; Tashiro, Yukihiro; Taniguchi, Masayuki; Sakai, Kenji.

In: Journal of Bioscience and Bioengineering, Vol. 117, No. 3, 01.03.2014, p. 318-324.

Research output: Contribution to journalArticle

Tongpim, Saowanit ; Meidong, Ratchanu ; Poudel, Pramod ; Yoshino, Satoshi ; Okugawa, Yuki ; Tashiro, Yukihiro ; Taniguchi, Masayuki ; Sakai, Kenji. / Isolation of thermophilic l-lactic acid producing bacteria showing homo-fermentative manner under high aeration condition. In: Journal of Bioscience and Bioengineering. 2014 ; Vol. 117, No. 3. pp. 318-324.
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