Isolation of two distinct cytochromes P-45011β with aldosterone synthase activity from bovine adrenocortical mitochondria

Tadashi Ogishima, Fumiko Mitani, Yuzuru Ishimura

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Two distinct forms of cytochrome P-45011β, with apparent molecular weights of 48,500 (48.5K) and 49, 500 (49.5K), have been isolated from bovine adrenocortical mitochondria. Their amino acid sequences up to the 19th position from the N-terminus were only different at the 6th position (Val and Ala for the 48.5K and 49.5K enzymes, respectively). Each sequence was assignable to a distinct cDNA clone for cytochrome P-45011β (Kirita, S., et al. [1988] J. Biochem. 104, 683-686), indicating that the two proteins originate from different genes in bovine adrenocortical cells. Both forms of cytochrome P-45011βwere capable of catalyzing aldosterone synthesis as well as the 11β- and 18-hydroxylation of 11-deoxycorticosterone. Thus, at least two distinct cytochrome P-45011β, species exist in the adrenal cortex and participate in steroidogenesis.

Original languageEnglish
Pages (from-to)497-499
Number of pages3
JournalJournal of biochemistry
Volume105
Issue number4
DOIs
Publication statusPublished - Jan 1 1989
Externally publishedYes

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Cytochrome P-450 CYP11B2
Mitochondria
Cytochromes
Desoxycorticosterone
Hydroxylation
Adrenal Cortex
Aldosterone
Amino Acid Sequence
Complementary DNA
Clone Cells
Genes
Molecular Weight
Molecular weight
Amino Acids
Enzymes
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Isolation of two distinct cytochromes P-45011β with aldosterone synthase activity from bovine adrenocortical mitochondria. / Ogishima, Tadashi; Mitani, Fumiko; Ishimura, Yuzuru.

In: Journal of biochemistry, Vol. 105, No. 4, 01.01.1989, p. 497-499.

Research output: Contribution to journalArticle

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