Two distinct forms of cytochrome P-45011β, with apparent molecular weights of 48,500 (48.5K) and 49, 500 (49.5K), have been isolated from bovine adrenocortical mitochondria. Their amino acid sequences up to the 19th position from the N-terminus were only different at the 6th position (Val and Ala for the 48.5K and 49.5K enzymes, respectively). Each sequence was assignable to a distinct cDNA clone for cytochrome P-45011β (Kirita, S., et al.  J. Biochem. 104, 683-686), indicating that the two proteins originate from different genes in bovine adrenocortical cells. Both forms of cytochrome P-45011βwere capable of catalyzing aldosterone synthesis as well as the 11β- and 18-hydroxylation of 11-deoxycorticosterone. Thus, at least two distinct cytochrome P-45011β, species exist in the adrenal cortex and participate in steroidogenesis.
|Number of pages||3|
|Journal||Journal of biochemistry|
|Publication status||Published - Jan 1 1989|
All Science Journal Classification (ASJC) codes
- Molecular Biology