Isolation of XAB2 complex involved in pre-mRNA splicing, transcription, and transcription-coupled repair

Isao Kuraoka, Shinsuke Ito, Tadashi Wada, Mika Hayashida, Lily Lee, Masafumi Saijo, Yoshimichi Nakatsu, Megumi Matsumoto, Tsukasa Matsunaga, Hiroshi Handa, Jun Qin, Yoshihiro Nakatani, Kiyoji Tanaka

Research output: Contribution to journalArticle

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Abstract

Nucleotide excision repair is a versatile repair pathway that counteracts the deleterious effects of various DNA lesions. In nucleotide excision repair, there is a transcription-coupled repair (TCR) pathway that focuses on DNA damage that blocks RNA polymerase IIo in transcription elongation. XAB2 (XPA-binding protein 2), containing tetratricopeptide repeats, has been isolated by virtue of its ability to interact with xeroderma pigmentosum group A protein (XPA). Moreover, XAB2 has been shown to interact with Cockayne syndrome group A and B proteins (CSA and CSB) and RNA polymerase II, as well as XPA, and is involved in TCR and transcription. Here we purified XAB2 as a multimeric protein complex consisting of hAquarius, XAB2, hPRP19, CCDC16, hISY1, and PPIE, which are involved in pre-mRNA splicing. Knockdown of XAB2 with small interfering RNA in HeLa cells resulted in a hypersensitivity to killing by UV light and a decreased recovery of RNA synthesis after UV irradiation and regular RNA synthesis. Enhanced interaction of XAB2 with RNA polymerase IIo or XPA was observed in cells treated with DNA-damaging agents, indicating DNA damage-responsive activity of the XAB2 complex. These results indicated that the XAB2 complex is a multifunctional factor involved in pre-mRNA splicing, transcription, and TCR.

Original languageEnglish
Pages (from-to)940-950
Number of pages11
JournalJournal of Biological Chemistry
Volume283
Issue number2
DOIs
Publication statusPublished - Jan 11 2008

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RNA Precursors
Transcription
Carrier Proteins
Repair
Cockayne Syndrome
DNA
DNA-Directed RNA Polymerases
DNA Repair
DNA Damage
Xeroderma Pigmentosum Group A Protein
Nucleotides
RNA
RNA Polymerase II
Ultraviolet Rays
HeLa Cells
Ultraviolet radiation
Small Interfering RNA
Elongation
Hypersensitivity
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Isolation of XAB2 complex involved in pre-mRNA splicing, transcription, and transcription-coupled repair. / Kuraoka, Isao; Ito, Shinsuke; Wada, Tadashi; Hayashida, Mika; Lee, Lily; Saijo, Masafumi; Nakatsu, Yoshimichi; Matsumoto, Megumi; Matsunaga, Tsukasa; Handa, Hiroshi; Qin, Jun; Nakatani, Yoshihiro; Tanaka, Kiyoji.

In: Journal of Biological Chemistry, Vol. 283, No. 2, 11.01.2008, p. 940-950.

Research output: Contribution to journalArticle

Kuraoka, I, Ito, S, Wada, T, Hayashida, M, Lee, L, Saijo, M, Nakatsu, Y, Matsumoto, M, Matsunaga, T, Handa, H, Qin, J, Nakatani, Y & Tanaka, K 2008, 'Isolation of XAB2 complex involved in pre-mRNA splicing, transcription, and transcription-coupled repair', Journal of Biological Chemistry, vol. 283, no. 2, pp. 940-950. https://doi.org/10.1074/jbc.M706647200
Kuraoka, Isao ; Ito, Shinsuke ; Wada, Tadashi ; Hayashida, Mika ; Lee, Lily ; Saijo, Masafumi ; Nakatsu, Yoshimichi ; Matsumoto, Megumi ; Matsunaga, Tsukasa ; Handa, Hiroshi ; Qin, Jun ; Nakatani, Yoshihiro ; Tanaka, Kiyoji. / Isolation of XAB2 complex involved in pre-mRNA splicing, transcription, and transcription-coupled repair. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 2. pp. 940-950.
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