Isolation of xylose-utilizing lactic acid bacteria from Ariake Sea - Note

Genta Kobayashi, Hiroyoshi Oka, Yukihiro Tashiro, Fumio Kato, Kohzo Kanda, Nobuyuki Hayashi

Research output: Contribution to journalArticlepeer-review

Abstract

A screening test was carried out to obtain lactic acid bacteria utilizing xylose from bottom sediments in Ariake Sea as sources. About 100 xylose-utilizing bacterial isolates as acid producer were obtained from several sources. More than 50 isolates were catalase-negative and eight isolates produced more than 8 g/l lactic acid in MRS medium containing xylose at 20 g/l. The OTK-1 strain, which produced a high concentration of lactic acid (more than 12 g/l) was isolated from tideland in Ariake Sea. The OTK-1 strain was rod-shaped, as observed under a microscope. This strain was designated as Lactobacillus brevis OTK-1 on the basis of 16S rDNA sequence analysis. The maximum yield of produced lactic acid with respect to utilized xylose, and the lactic acid productivity were 1.15 mol/mol and 1.0 g/l/h, respectively. This strain was able to utilize xylooligosaccharide as a fermentative substrate.

Original languageEnglish
Pages (from-to)217-220
Number of pages4
JournalSeibutsu-kogaku Kaishi
Volume86
Issue number5
Publication statusPublished - Jul 8 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology

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