Isotachophoretic Examination of Interaction of Intercalators with Ribodinucleoside Monophosphates

Kunihiko Dohtsu, Shigeori Takenaka, Makoto Takagi, Naotoshi Nakashima

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    3 Citations (Scopus)


    The isotachophoretic behavior of ribodinucleoside monophosphates (XpY) was studied in aqueous solution in the presence of intercalators under neutral pH conditions. The interaction of intercalators with the ribodinucleoside monophosphates was studied by the electrophoretic retardation of the nucleotides. Among the acridine-type intercalators, proflavine and quinacrine interacted favorably with ribodinucleoside monophosphates following the order CpG>GpC>UpA, while 9-aminoacridine interacted in the order UpA>GpC=CpG. Anthraquinone-type intercalators behaved similarly, among these, l, 5-bis[(diethylaminoethyl)amino]anthraquinone Interacted with the nucleotides more strongly than did l, 8-bis[(diethylaminoethyl)amino]anthraquinone. Use of isotachophoresis is discussed as a rapid and convenient method for the characterization of DNA intercalators.

    Original languageEnglish
    Pages (from-to)251-254
    Number of pages4
    Journalanalytical sciences
    Issue number3
    Publication statusPublished - Jan 1 1988

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry


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