Japanese cedar pollinosis and HLA-DP5

T. Hori, N. Kamikawaji, A. Kimura, T. Sone, N. Komiyama, S. Komiyama, T. Sasazuki

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Japanese cedar pollinosis is a type I allergic disease caused by Japanese cedar (Cryptomeria japonica) pollen. We investigated the association between the disease and HLA class II alleles by HLA-DNA typing using a PCR-SSOP method and found that the frequency of HLA-DP5 (DPA1(*)02022 and DPB1(*)0501) was significantly increased in the patients. To investigate whether the HLA-DP5 molecule is directly involved in the pathogenesis of the disease, Japanese cedar pollen antigen (CPAg)-specific T cell lines were established from 3 patients who possessed HLA-DP5 (DPA1(*)02022/ DPB1(*)0501). By using these CPAg-specific T cell lines and HLA class II-expressing L-cell transfectants, we found that disease-associated HLA-DP5 restricted T cells specific for CPAg existed in the patients. Furthermore, among 38 synthesized overlapping peptides spanning the entire length of one of the major Japanese cedar pollen allergens, Cry j 1, an immunodominant peptide which induced HLA-DP5 restricted Th2 was identified. These observations suggest that the HLA-DP5 may be involved, at least in part, in the pathogenesis, by helping the IgE antibody production against CPAg.

Original languageEnglish
Pages (from-to)485-491
Number of pages7
JournalTissue antigens
Volume47
Issue number6
DOIs
Publication statusPublished - 1996

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Genetics

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