JMJD8 Regulates Angiogenic Sprouting and Cellular Metabolism by Interacting with Pyruvate Kinase M2 in Endothelial Cells

Jes Niels Boeckel, Anja Derlet, Simone F. Glaser, Annika Luczak, Tina Lucas, Andreas W. Heumüller, Marcus Krüger, Christoph M. Zehendner, David Kaluza, Anuradha Doddaballapur, Kisho Ohtani, Karine Treguer, Stefanie Dimmeler

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective-Jumonji C (JmjC) domain-containing proteins modify histone and nonhistone proteins thereby controlling cellular functions. However, the role of JmjC proteins in angiogenesis is largely unknown. Here, we characterize the expression of JmjC domain-containing proteins after inducing endothelial differentiation of murine embryonic stem cells and study the function of JmjC domain-only proteins in endothelial cell (EC) functions. Approach and Results-We identified a large number of JmjC domain-containing proteins regulated by endothelial differentiation of murine embryonic stem cells. Among the family of JmjC domain-only proteins, Jmjd8 was significantly upregulated on endothelial differentiation. Knockdown of Jmjd8 in ECs significantly decreased in vitro network formation and sprouting in the spheroid assay. JMJD8 is exclusively detectable in the cytoplasm, excluding a function as a histone-modifying enzyme. Mass spectrometry analysis revealed JMJD8-interacting proteins with known functions in cellular metabolism like pyruvate kinase M2. Accordingly, knockdown of pyruvate kinase M2 in human umbilical vein ECs decreased endothelial sprouting in the spheroid assay. Knockdown of JMJD8 caused a reduction of EC metabolism as measured by Seahorse Bioscience extracellular flux analysis. Conversely, overexpression of JMJD8 enhanced cellular oxygen consumption rate of ECs, reflecting an increased mitochondrial respiration. Conclusions-Jmjd8 is upregulated during endothelial differentiation and regulates endothelial sprouting and metabolism by interacting with pyruvate kinase M2.

Original languageEnglish
Pages (from-to)1425-1433
Number of pages9
JournalArteriosclerosis, thrombosis, and vascular biology
Volume36
Issue number7
DOIs
Publication statusPublished - Jul 1 2016

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Pyruvate Kinase
Endothelial Cells
Embryonic Stem Cells
Histones
Smegmamorpha
Umbilical Veins
Protein C
Oxygen Consumption
Mass Spectrometry
Respiration
Cytoplasm
Proteins
Protein Domains
Enzymes

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

JMJD8 Regulates Angiogenic Sprouting and Cellular Metabolism by Interacting with Pyruvate Kinase M2 in Endothelial Cells. / Boeckel, Jes Niels; Derlet, Anja; Glaser, Simone F.; Luczak, Annika; Lucas, Tina; Heumüller, Andreas W.; Krüger, Marcus; Zehendner, Christoph M.; Kaluza, David; Doddaballapur, Anuradha; Ohtani, Kisho; Treguer, Karine; Dimmeler, Stefanie.

In: Arteriosclerosis, thrombosis, and vascular biology, Vol. 36, No. 7, 01.07.2016, p. 1425-1433.

Research output: Contribution to journalArticle

Boeckel, JN, Derlet, A, Glaser, SF, Luczak, A, Lucas, T, Heumüller, AW, Krüger, M, Zehendner, CM, Kaluza, D, Doddaballapur, A, Ohtani, K, Treguer, K & Dimmeler, S 2016, 'JMJD8 Regulates Angiogenic Sprouting and Cellular Metabolism by Interacting with Pyruvate Kinase M2 in Endothelial Cells', Arteriosclerosis, thrombosis, and vascular biology, vol. 36, no. 7, pp. 1425-1433. https://doi.org/10.1161/ATVBAHA.116.307695
Boeckel, Jes Niels ; Derlet, Anja ; Glaser, Simone F. ; Luczak, Annika ; Lucas, Tina ; Heumüller, Andreas W. ; Krüger, Marcus ; Zehendner, Christoph M. ; Kaluza, David ; Doddaballapur, Anuradha ; Ohtani, Kisho ; Treguer, Karine ; Dimmeler, Stefanie. / JMJD8 Regulates Angiogenic Sprouting and Cellular Metabolism by Interacting with Pyruvate Kinase M2 in Endothelial Cells. In: Arteriosclerosis, thrombosis, and vascular biology. 2016 ; Vol. 36, No. 7. pp. 1425-1433.
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abstract = "Objective-Jumonji C (JmjC) domain-containing proteins modify histone and nonhistone proteins thereby controlling cellular functions. However, the role of JmjC proteins in angiogenesis is largely unknown. Here, we characterize the expression of JmjC domain-containing proteins after inducing endothelial differentiation of murine embryonic stem cells and study the function of JmjC domain-only proteins in endothelial cell (EC) functions. Approach and Results-We identified a large number of JmjC domain-containing proteins regulated by endothelial differentiation of murine embryonic stem cells. Among the family of JmjC domain-only proteins, Jmjd8 was significantly upregulated on endothelial differentiation. Knockdown of Jmjd8 in ECs significantly decreased in vitro network formation and sprouting in the spheroid assay. JMJD8 is exclusively detectable in the cytoplasm, excluding a function as a histone-modifying enzyme. Mass spectrometry analysis revealed JMJD8-interacting proteins with known functions in cellular metabolism like pyruvate kinase M2. Accordingly, knockdown of pyruvate kinase M2 in human umbilical vein ECs decreased endothelial sprouting in the spheroid assay. Knockdown of JMJD8 caused a reduction of EC metabolism as measured by Seahorse Bioscience extracellular flux analysis. Conversely, overexpression of JMJD8 enhanced cellular oxygen consumption rate of ECs, reflecting an increased mitochondrial respiration. Conclusions-Jmjd8 is upregulated during endothelial differentiation and regulates endothelial sprouting and metabolism by interacting with pyruvate kinase M2.",
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AU - Glaser, Simone F.

AU - Luczak, Annika

AU - Lucas, Tina

AU - Heumüller, Andreas W.

AU - Krüger, Marcus

AU - Zehendner, Christoph M.

AU - Kaluza, David

AU - Doddaballapur, Anuradha

AU - Ohtani, Kisho

AU - Treguer, Karine

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N2 - Objective-Jumonji C (JmjC) domain-containing proteins modify histone and nonhistone proteins thereby controlling cellular functions. However, the role of JmjC proteins in angiogenesis is largely unknown. Here, we characterize the expression of JmjC domain-containing proteins after inducing endothelial differentiation of murine embryonic stem cells and study the function of JmjC domain-only proteins in endothelial cell (EC) functions. Approach and Results-We identified a large number of JmjC domain-containing proteins regulated by endothelial differentiation of murine embryonic stem cells. Among the family of JmjC domain-only proteins, Jmjd8 was significantly upregulated on endothelial differentiation. Knockdown of Jmjd8 in ECs significantly decreased in vitro network formation and sprouting in the spheroid assay. JMJD8 is exclusively detectable in the cytoplasm, excluding a function as a histone-modifying enzyme. Mass spectrometry analysis revealed JMJD8-interacting proteins with known functions in cellular metabolism like pyruvate kinase M2. Accordingly, knockdown of pyruvate kinase M2 in human umbilical vein ECs decreased endothelial sprouting in the spheroid assay. Knockdown of JMJD8 caused a reduction of EC metabolism as measured by Seahorse Bioscience extracellular flux analysis. Conversely, overexpression of JMJD8 enhanced cellular oxygen consumption rate of ECs, reflecting an increased mitochondrial respiration. Conclusions-Jmjd8 is upregulated during endothelial differentiation and regulates endothelial sprouting and metabolism by interacting with pyruvate kinase M2.

AB - Objective-Jumonji C (JmjC) domain-containing proteins modify histone and nonhistone proteins thereby controlling cellular functions. However, the role of JmjC proteins in angiogenesis is largely unknown. Here, we characterize the expression of JmjC domain-containing proteins after inducing endothelial differentiation of murine embryonic stem cells and study the function of JmjC domain-only proteins in endothelial cell (EC) functions. Approach and Results-We identified a large number of JmjC domain-containing proteins regulated by endothelial differentiation of murine embryonic stem cells. Among the family of JmjC domain-only proteins, Jmjd8 was significantly upregulated on endothelial differentiation. Knockdown of Jmjd8 in ECs significantly decreased in vitro network formation and sprouting in the spheroid assay. JMJD8 is exclusively detectable in the cytoplasm, excluding a function as a histone-modifying enzyme. Mass spectrometry analysis revealed JMJD8-interacting proteins with known functions in cellular metabolism like pyruvate kinase M2. Accordingly, knockdown of pyruvate kinase M2 in human umbilical vein ECs decreased endothelial sprouting in the spheroid assay. Knockdown of JMJD8 caused a reduction of EC metabolism as measured by Seahorse Bioscience extracellular flux analysis. Conversely, overexpression of JMJD8 enhanced cellular oxygen consumption rate of ECs, reflecting an increased mitochondrial respiration. Conclusions-Jmjd8 is upregulated during endothelial differentiation and regulates endothelial sprouting and metabolism by interacting with pyruvate kinase M2.

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