Judgment of marijuana pollen by using anti-marijuana pollen allergen IgE antibody and anti-THCA monoclonal antibody

Yukihiro Shoyama, Hiroyuki Tanaka

Research output: Contribution to journalArticle


We established an ELISA for marihuana pollens using anti-marihuana pollen allergen IgE. This ELISA distinguished only the marihuana pollen from various plant pollens. When the marihuana pollens on PVDF membrane were treated with 0.125 M NaHCO(J), allergens appeared and fixed on the membrane. A single pollen can be detected by the anti-marihuana pollen alergen IgE under microscope. It became evident that marihuana pollens contained cannabinoids which were determined by ELISA using anti-THCA MAb.

Original languageEnglish
Pages (from-to)110-111
Number of pages2
JournalJapanese Journal of Forensic Toxicology
Issue number2
Publication statusPublished - Sep 6 1999


All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Toxicology

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