JunB plays a crucial role in development of regulatory T cells by promoting IL-2 signaling

Takaharu Katagiri, Soh Yamazaki, Yuto Fukui, Kotaro Aoki, Hideo Yagita, Takashi Nishina, Tetuo Mikami, Sayaka Katagiri, Ayako Shiraishi, Soichiro Kimura, Kazuhiro Tateda, Hideki Sumimoto, Shogo Endo, Hideto Kameda, Hiroyasu Nakano

Research output: Contribution to journalArticle

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Abstract

The AP-1 transcription factor JunB plays crucial roles in multiple biological processes, including placental formation and bone homeostasis. We recently reported that JunB is essential for development of Th17 cells, and thus Junb-deficient mice are resistant to experimental autoimmune encephalomyelitis. However, the role of JunB in CD4+ T cells under other inflammatory disease conditions is unknown. Here we show that mice lacking JunB in CD4+ T cells (Junbfl/flCd4-Cre mice) were more susceptible to dextran sulfate sodium (DSS)-induced colitis because of impaired development of regulatory T (Treg) cells. Production of interleukin (IL)-2 and expression of CD25, a high affinity IL-2 receptor component, were decreased in Junb-deficient CD4+ T cells in vitro and in vivo. Naive CD4+ T cells from Junbfl/flCd4-Cre mice failed to differentiate into Treg cells in the absence of exogenously added IL-2 in vitro. A mixed bone marrow transfer experiment revealed that defective Treg development of Junb-deficient CD4+ T cells was not rescued by co-transferred wild-type cells, indicating a significance of the cell-intrinsic defect. Injection of IL-2-anti-IL-2 antibody complexes induced expansion of Treg cells and alleviated DSS-induced colitis in Junbfl/flCd4-Cre mice. Thus JunB plays a crucial role in the development of Treg cells by facilitating IL-2 signaling.

Original languageEnglish
Pages (from-to)1104-1117
Number of pages14
JournalMucosal Immunology
Volume12
Issue number5
DOIs
Publication statusPublished - Sep 1 2019

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Regulatory T-Lymphocytes
Interleukin-2
T-Lymphocytes
Dextran Sulfate
Colitis
Biological Phenomena
Th17 Cells
Autoimmune Experimental Encephalomyelitis
Interleukin-2 Receptors
Transcription Factor AP-1
Osteogenesis
Homeostasis
Bone Marrow
Injections
Antibodies
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Katagiri, T., Yamazaki, S., Fukui, Y., Aoki, K., Yagita, H., Nishina, T., ... Nakano, H. (2019). JunB plays a crucial role in development of regulatory T cells by promoting IL-2 signaling. Mucosal Immunology, 12(5), 1104-1117. https://doi.org/10.1038/s41385-019-0182-0

JunB plays a crucial role in development of regulatory T cells by promoting IL-2 signaling. / Katagiri, Takaharu; Yamazaki, Soh; Fukui, Yuto; Aoki, Kotaro; Yagita, Hideo; Nishina, Takashi; Mikami, Tetuo; Katagiri, Sayaka; Shiraishi, Ayako; Kimura, Soichiro; Tateda, Kazuhiro; Sumimoto, Hideki; Endo, Shogo; Kameda, Hideto; Nakano, Hiroyasu.

In: Mucosal Immunology, Vol. 12, No. 5, 01.09.2019, p. 1104-1117.

Research output: Contribution to journalArticle

Katagiri, T, Yamazaki, S, Fukui, Y, Aoki, K, Yagita, H, Nishina, T, Mikami, T, Katagiri, S, Shiraishi, A, Kimura, S, Tateda, K, Sumimoto, H, Endo, S, Kameda, H & Nakano, H 2019, 'JunB plays a crucial role in development of regulatory T cells by promoting IL-2 signaling', Mucosal Immunology, vol. 12, no. 5, pp. 1104-1117. https://doi.org/10.1038/s41385-019-0182-0
Katagiri T, Yamazaki S, Fukui Y, Aoki K, Yagita H, Nishina T et al. JunB plays a crucial role in development of regulatory T cells by promoting IL-2 signaling. Mucosal Immunology. 2019 Sep 1;12(5):1104-1117. https://doi.org/10.1038/s41385-019-0182-0
Katagiri, Takaharu ; Yamazaki, Soh ; Fukui, Yuto ; Aoki, Kotaro ; Yagita, Hideo ; Nishina, Takashi ; Mikami, Tetuo ; Katagiri, Sayaka ; Shiraishi, Ayako ; Kimura, Soichiro ; Tateda, Kazuhiro ; Sumimoto, Hideki ; Endo, Shogo ; Kameda, Hideto ; Nakano, Hiroyasu. / JunB plays a crucial role in development of regulatory T cells by promoting IL-2 signaling. In: Mucosal Immunology. 2019 ; Vol. 12, No. 5. pp. 1104-1117.
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