Juvenile hormone titre and related gene expression during the change of reproductive modes in the pea aphid

A. Ishikawa, K. Ogawa, H. Gotoh, T. K. Walsh, D. Tagu, J. A. Brisson, C. Rispe, S. Jaubert-Possamai, T. Kanbe, T. Tsubota, T. Shiotsuki, T. Miura

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Most aphids show reproductive polyphenism, i.e. they alternate their reproductive modes from parthenogenesis to sexual reproduction in response to short photoperiods. Although juvenile hormone (JH) has been considered a likely candidate for regulating the transition from asexual to sexual reproduction after photoperiod sensing, there are few studies investigating the direct relationship between JH titres and the reproductive-mode change. In addition, the sequencing of the pea aphid genome has allowed identification of the genes involved in the JH pathway, which in turn allows us to examine their expression levels in relation to the reproductive-mode change. Using liquid chromatography-mass spectrometry in the pea aphid, JHIII titre was shown to be lower in aphids producing sexual morphs under short-day conditions than in aphids producing parthenogenetic morphs under long-day conditions. The expression levels of genes upstream and downstream of JH action were quantified by real-time quantitative reverse-transcription-PCR across the reproductive-mode change. The expression level of JH esterase, which is responsible for JH degradation, was significantly higher in aphids reared under short-day conditions. This suggests that the upregulation of the JH degradation pathway may be responsible for the lower JHIII titre in aphids exposed to short-days, leading to the production of sexual morphs.

Original languageEnglish
Pages (from-to)49-60
Number of pages12
JournalInsect Molecular Biology
Volume21
Issue number1
DOIs
Publication statusPublished - Feb 1 2012
Externally publishedYes

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Juvenile Hormones
Aphids
Acyrthosiphon pisum
Peas
juvenile hormones
Aphidoidea
Gene Expression
gene expression
morphs
sexual reproduction
Photoperiod
photoperiod
Reproduction
juvenile hormone esterase
Parthenogenesis
degradation
parthenogenesis
liquid chromatography
Liquid Chromatography
genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Insect Science

Cite this

Juvenile hormone titre and related gene expression during the change of reproductive modes in the pea aphid. / Ishikawa, A.; Ogawa, K.; Gotoh, H.; Walsh, T. K.; Tagu, D.; Brisson, J. A.; Rispe, C.; Jaubert-Possamai, S.; Kanbe, T.; Tsubota, T.; Shiotsuki, T.; Miura, T.

In: Insect Molecular Biology, Vol. 21, No. 1, 01.02.2012, p. 49-60.

Research output: Contribution to journalArticle

Ishikawa, A, Ogawa, K, Gotoh, H, Walsh, TK, Tagu, D, Brisson, JA, Rispe, C, Jaubert-Possamai, S, Kanbe, T, Tsubota, T, Shiotsuki, T & Miura, T 2012, 'Juvenile hormone titre and related gene expression during the change of reproductive modes in the pea aphid', Insect Molecular Biology, vol. 21, no. 1, pp. 49-60. https://doi.org/10.1111/j.1365-2583.2011.01111.x
Ishikawa, A. ; Ogawa, K. ; Gotoh, H. ; Walsh, T. K. ; Tagu, D. ; Brisson, J. A. ; Rispe, C. ; Jaubert-Possamai, S. ; Kanbe, T. ; Tsubota, T. ; Shiotsuki, T. ; Miura, T. / Juvenile hormone titre and related gene expression during the change of reproductive modes in the pea aphid. In: Insect Molecular Biology. 2012 ; Vol. 21, No. 1. pp. 49-60.
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