TY - JOUR
T1 - Kainic acid-induced seizures in the common marmoset
AU - Ishikawa, Akiyoshi
AU - Mizuno, Yuri
AU - Sakai, Keita
AU - Maki, Takehiro
AU - Tanaka, Ryo
AU - Oda, Yasuhiro
AU - Niimi, Kimie
AU - Takahashi, Eiki
N1 - Publisher Copyright:
© 2020 Elsevier Inc.
PY - 2020/5/7
Y1 - 2020/5/7
N2 - Treatment of epilepsy remains difficult because patients suffer from pharmacoresistant forms of the disease and drug side-effects. Thus, there is an urgent need to identify not only new antiepileptic drug candidates but also novel epileptic animal models. Here, we characterize seizures induced with kainic acid (KA) in the common marmoset (Callithrix jacchus). Adult marmosets received 0.1, 1, or 10 mg/kg of KA subcutaneously. All animals exhibited early convulsive behavior (seizure scores of I and II on the Racine scale). Seizure scores were low at lower KA doses, but the highest dose of KA tested triggered generalized seizures (scores IV and V on the Racine scale). We next performed preliminary evaluation of the efficacy of the antiepileptic drug diazepam. This drug at 1 mg/kg (delivered subcutaneously) prevented 10 mg/kg KA-induced stage V seizures. KA administration to marmosets reliably triggers generalized seizures; therefore, the marmoset is a useful animal model in which to analyze the seizures of a nonhuman primate brain and to develop new treatments for epilepsy.
AB - Treatment of epilepsy remains difficult because patients suffer from pharmacoresistant forms of the disease and drug side-effects. Thus, there is an urgent need to identify not only new antiepileptic drug candidates but also novel epileptic animal models. Here, we characterize seizures induced with kainic acid (KA) in the common marmoset (Callithrix jacchus). Adult marmosets received 0.1, 1, or 10 mg/kg of KA subcutaneously. All animals exhibited early convulsive behavior (seizure scores of I and II on the Racine scale). Seizure scores were low at lower KA doses, but the highest dose of KA tested triggered generalized seizures (scores IV and V on the Racine scale). We next performed preliminary evaluation of the efficacy of the antiepileptic drug diazepam. This drug at 1 mg/kg (delivered subcutaneously) prevented 10 mg/kg KA-induced stage V seizures. KA administration to marmosets reliably triggers generalized seizures; therefore, the marmoset is a useful animal model in which to analyze the seizures of a nonhuman primate brain and to develop new treatments for epilepsy.
UR - http://www.scopus.com/inward/record.url?scp=85080101104&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85080101104&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2020.02.121
DO - 10.1016/j.bbrc.2020.02.121
M3 - Article
C2 - 32115153
AN - SCOPUS:85080101104
VL - 525
SP - 595
EP - 599
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -